Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

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94 articles

Anticancer Activity of Oridonin Against Esophageal Cancer Cells Enhanced by Special Electromagnetic Field Treated Water

Jiang PI, Jin-huan JIANG, Hua JIN, Xin WANG, Yuan PENG, Ji-ye CAI
Oridonin, the main pharmacological active substance isolated from Rabdosia Rubescens, has been found to show anti-inflammatory, pro-apoptotic, neurological effects, and excellent anticancer activities. However, the clinical application of oridonin is still limited by its poor solubility. The special...

Study on Animal Model Characterization of Chinese Medicine

Xu ZHANG, Hui ZHOU, Xuan-xuan ZHU, Yi LIANG, Jia-chen WANG, Yan-yan MIAO, Ming-san MIAO
Characterization can reflect objectivity and represent objective things. With the development of pharmacology of Chinese Medicine in recent years and the deepening of animal model research in Chinese medicine, how to accurately and quickly copy the successful model animals according to the characterization...

Modern Analysis of Traditional Chinese Medicine for Chronic Abacterial Prostatitis

Jing LIU, Tan WANG, Huan LI, Ming BAI, Ming-san MIAO
Objective: To explore the therapeutic principles of modern Chinese medicine for chronic prostatitis and the application of TCM in CNP. Procedures and Methods: Analysised the relevant information and literature, to explore the mechanism of Chinese medicine for the treatment of prostatitis, sum up TCM...

Researches on the Progress of Antiallergic Traditional Chinese Medicine and Its Mechanism

Xuan WU
With the study of anti-allergy medicine, it proves traditional Chinese medicine has anti-allergic effects and several other functions, such as protecting and stabilizing the target cell membrane (reduce or prevent degranulation, release the allergy mediators, increase the intracellular cAMP levels),...

The Significance of Restoring the Traditional Diet of Chinese People from the High Incidence of Modern Diseases

Bao-song LIU, Meng-fan PENG, Yan-yan MIAO, Ming BAI, Ming-San MIAO
From the reform and opening up in 1979 to the year of twenty-first Century, China's economy has been growing rapidly,and great changes have taken place in people's traditional eating habits. Gradually westernized modern diet has become one of the main reasons ,which leading the incidence of many chronic...

Analysis of Animal Models of Alopecia Areata(AA) Based on Clinical Features of Traditional Chinese and Western Medicine

Huan LI, Ming BAI, Yan-yan MIAO, Xiao-yan FANG, Ming-san MIAO
Objective: Analysis of alopecia areata animal model production methods, characteristics and characteristics of Chinese and Western alopecia areata anastomosis. Methods: To summarize the characteristics of the alopecia areata animal model, according to alopecia areata diagnosis and clinical characteristics...

Study on the Key Technology of Micro-arc Oxidation of TA2 Titanium for Medical Use

Yu-lei LI, Yue-lai DAI, Jin-jun TANG, Xiao-yu JIN, Qun WANG
By micro-arc oxidation technology, the TA2 titanium metal surface modification, by scanning electron microscopy (SEM) experiments, the effects of different voltage parameters on film thickness and surface microstructure were studied. The results show that as the voltage increases and the oxidation time...

Photosynthetic Characteristics of Marestail Using PAM Fluorometry

Li-nan GAO
In this work, we used PAM fluorometry to examine photosynthetic characteristics of marestail (Hippuris vulgaris) across a water temperature gradient in three lakes. Three lakes were studied across a gradient in water temperature, with low water temperature conditions in Grass Lake and Arrow Bamboo Lake,...

Antitumor Effect and Immune Regulation of the Pingyan Capsule on Lewis Lung Cancer Cells in Vivo

Na LI, Yan LI, Tian-yu LIU
Objective: we investigated the function of Pingyan capsule on antitumor and immune regulation in Lewis lung cancer tumor-bearing mice. Methods: mice were randomly divided into normal control group, model group, positive control group, high, medium and low dose Pingyan capsule groups. Lewis lung carcinoma...

ITS andTrnl-F Sequences Analysis of Pulsatilla Chinensis (Bge.) Regel

Shao-xuan ZHANG, Guang-zhu LIN, Tian-tian WANG, Ya-rong LI
To provide scientific data of the internal transcribed spacer (ITS) and trnL-F sequences for the authentication of Pulsatilla chinesis(Bge.) Regel., we extracted the genome DNA from the leaves of Pulsatilla chinesis(Bge.) Regel., collected in Jilin Province, amplified the ITS and trnL-F region using...

Transurethral Plasma Cutting and Prostate Tear in Addition to the Treatment of Hyperplasia of Prostate the Clinical Efficacy of Comparison

Fan-ping MENG, Di ZOU, Yan HE, Ji GAO
Objective To compare the efficacy and complications between bipolar plasmakinetic resection of prostate and bipolar plasmakineticenucleation of prostate for benign prostatic hyperplasia(BPH). Methods 303 cases of BPH were divided into 2 groups, 143 cases aged (73ñ13.4) yearswith prostate volume of (76.5ñ22.5)ml...

Analysis of Cytoplasm Polymorphism on the TR2 Locus of Mitochondria Genome in Leaf Beet Line SK-5

Qiao-hong LIU, Li-ping LIU, Cheng-fei LUO, Da-you CHENG, Cui-hong DAI, Shu-zhi SHI, Nai-guo LIANG, Tian-jiao LIU
One mitochondrial minisatellite locus was used to study cytoplasmic diversity in leaf beet lines SK-5 population from Chongqing, China. The analysis of one mitochondrial minisatellite locus (TR2) was conducted using variable number of tandem repeats (VNTRs) molecular markers method within 50 single plants...

Review and Outlook of Stem Cell Transplantation for Neonatal Hypoxic- Ischemic Encephalopathy

Qiu-li WANG, Hong-yan LV, Peng-shun REN, Lian-xiang LI
Purpose: To research the states and feasibility of stem cells for neonatal hypoxic ischemic encephalopathy (HIE). Methods: In this study, "newborn, hypoxic ischemic encephalopathy, pathogenesis, stem cell transplantation" as the key words, which are searched the papers published in CNKI database and...

Expression of NOB1 Gene and Its Clinical Significance in Ovarian Cancer

Ning LIU, Shun-qing ZHOU, Yang LIN
[Purposes] To explore the correlation between mRNA expression of NOB1 gene in tissues from ovarian cancer, normal ovary, benign ovarian tumor. [Methods] 50 samples of ovarian cancer as the experimental group, 20 samples of normal ovarian tissues and 50 samples of benign ovarian tumor as control groups....

A Food-Grade, Secretory Expression of IL1RA Protein in a Lactococcus Lactis System for Potential Oral Therapy on IBD

Jin CHENG, Jin CAO, Jia CAO, Xiao-feng WANG, Yan-ru GE
Interleukin 1 (IL1 ) is a potential target for IBD treatment. IL1 receptor antagonist (IL1RA) can blocks the biologic activity of IL1 by competitively inhibiting IL1 binding to the IL1R, so IL1RA is suggested to have possible therapeutic applications in treatment of IBD. By using a food-grade recombinant...

Development of a FES System for Hemiplegic Rehabilitation Based on Patient Healthy Limb's EMG Signal

Zhan ZENG, Chang-jun LI, Chun-bao WANG, Quan-quan LIU, Li-hong DUAN, Tong-yang SUN, Qing SHI, Zhuo-hua LIN, Ya-jing SHEN, Wan-feng SHANG, Zheng-zhi WU
Thisÿpaperÿpresents a functional electrical stimulation system to mimic the EMG characterization of patient healthy limb to exercise his/her affected limb. Firstly, the existing problems of functional electrical stimulation system are analyzed. Generally, the existing electrical stimulation systems suffer...

Clinical Application of Soft Ureter Mirror

Fan-Ping MENG, Yong-liang CUI, Jun WEI, Ji GAO
Objective:To summarize the clinical application of ureter soft mirror. Methods:The treatment of urinary calculi includes ESWL, percutaneous nephrolithotomy, ureter soft mirror and so on , The treatment of urinary calculi with ureter soft mirror is becoming more and more widespread, and the indications...

Preliminary Study of LAMP Method for the Detection of Equine Infectious Anemia Virus

Jia-qi HAN, Han ZHANG, Qin TANG, Ting-chuan LIU, Jing XU, Li -rui LI, Gang YU, Yin WANG, Xue-ping YAO, Ze-xiao YANG
To develop a rapid molecular biological method for the detection of equine infectious anemia virus (EIAV), a set of 4 primers were designed according to the conserved sequence fragment of the gag non-structure protein gene of EIAV published in GenBank, and the reverse transcriptase loop-mediated isothermal...

The Harm of Fog and Thick Haze to the Body and the Prevention of Chinese Medicine

Le KANG, Yan-yan MIAO, Ming-san MIAO, Zhen-ya HU
In recent years, The situation that China's fog and thick haze pollution is very grim, which has threatened people's health and daily life work. fog and thick haze is a meteorological phenomenon, which is the essence of human activities caused by fine particulate pollution. With the increase in the severity...

Characterization of a Serine Carboxypeptidase Hoscp from Holotrichia Oblita Faldermann (Coleoptera: Scarabaeoidea)

Dan ZHAO, Ya-kun ZHANG, Xiao-ping YAN, Wei GUO, Xiao-min LIU
In this report, we have identified a novel serine carboxypeptidase, named HoSCP, from the midgut of a destructive agricultural and landscape pest, Holotrichia oblita. HoSCP cDNA gene was cloned by immunoscreening method. Sequence analysis showed about 49kDa HoSCP consists of 457 amino acid residues with...

Bioink of Multi-Biomaterials with Tyrosine Reinforcement for 3D Bioprinting of Skin Constructs

Ke YANG, Yong SHI, Hong-bo ZHANG, Rui-xue YIN, Shih-mo YANG, Wen-jun ZHANG
In this paper, a bioink composed of multiple native materials with tyrosine micro-particle reinforcement for 3D bioprinting of skin scaffolds was developed. The rheological property of the developed bioink was optimized for 3D Bioprinting process. The results showed that Tyrosine micro-particle reinforcement...

Isolation and Preliminary Molecular Characterization of Novel Anaerobic Microbial Species from the Interface Between Zaopei (Fermented Grains) and the Bottom Pit Mud

Ying HAN, Hong-kui HE, Hang DU, Run-jie CAO, Hai-ning NING, Zhi-hui TANG, An-jun LI, Zhi-zhou ZHANG
Strong-aroma type liquor occupies over 70% of Chinese liquor market, but the namelist of key microbes responsible for liquor flavor is still vague. Lots of data indicate that the interface between the bottom pit mud and Zaopei (fermented grains) plays an important role for aroma molecule production,...

Antimicrobial Activities of Polyphenol Extract of Peanut Skins

Wen-xu XIA, Ping ZHAO, Jia-bin WANG, Zhan-juan LI, Nanju Alice LEE
In this study, the cardinal and purple peanut testa were used as the raw materials for extracting polyphenol substances with ultrasonic and freeze-drying. Then the bacteriostasis and antifungal activities of the extracts were investigated by the methods of disc diffussion and equivalent against the Benzylpenicillin...

Analysis of Chronic Skin Ulcer Model Based on Clinical Features of Traditional Chinese Medicine and Western Medicine

Ming-san MIAO, Xiao-li YAN, Ming BAI, Yan-yan MIAO
Objective: Analysis anastomosis of the commonly used animal models and clinical features of chronic skin ulcer disease, put forward to recommendations for model refinement. Method: Summarize the characteristics of existing chronic skin ulcer model and molding method, according to traditional Chinese...

Ureteroscopy Holmium Laser Lithotripsy Treatment Complicated Ureteral Stones

Fan-ping MENG, Jun WEI, Yong-liang CUI, Ji GAO
Objective: to discuss the effectiveness and safety of the ureteral stone in complicated ureteroscope of ureteroscope. Methods: in this study, 47 cases of ureteral calculi were treated by the ureteroscope of the ureteroscope, which was collected from February 2015 to February 2017. Results: in 47 cases,...

Improved Camptothecin Production of Camptotheca Acuminata on Hydroponic Medium Supplemented with Various Chemicals

Qing-qian ZENG, Lin JIANG, Long-ping ZHU, Hai ZHENG, De-po YANG
A Hydroponic culture was conducted to determine the effect of organic acids and metal ions on plant growth, camptothecin concentrations and yield of Camptotheca acuminata, in an effort to increase the camptothecin yield by artificial cultivation. For maximal camptothecin production, increase of maleic...

Twin-Studies on the Role of Non-Genetic Factors in T1D Pathogenesis

Li-li MA, Yan-ling WU, Ying DONG, Yoshimasa TANAKA, Wen ZHANG
Type 1 diabetes (T1D) is a complex autoimmune disease characterized by T cell-mediated destruction of pancreatic cells, which is resulted from the interaction of genetic and environmental factors. Twin-study is one of the most important methods to identify the genetic basis of complex diseases or traits...

The Protection of the Patients' Privacy in Anesthesiology Clinic

Na WANG, Jin-guo WANG
Patients will fall asleep under anesthesiology in most cases, so they will lose their consciousness to protect themselves. Therefore, the anesthetists carry on more duties to protect the patients' privacy.

A Comparison of the Special Wetting Property between Locust and Butterfly Wing

Yan FANG, Gang SUN, Jing-shi YIN, Wan-xing WANG, Yu-qian WANG
The microstructure, wettability and chemical composition of butterfly and locust wing surfaces were investigated by a scanning electron microscope (SEM), a contact angle meter and a Fourier transform infrared spectrometer (FT-IR). The hydrophobicity models were established on the basis of Cassie equation....

Indirect Spectrophotometric Determination of Acetylcysteine in Pharmaceutical Sample using Fe(III)-Sulfosalicylic Acid System

Xin-rong WEN, Chang-qing TU
In acidic medium, Fe (III) can be reduced to Fe (II) by hydrosulfuryl (-SH) in acetylcysteine molecule, and then using sulfosalicylic acid as chromogenic reagent of Fe (III) to determinate the acetylcysteine indirectly by discoloration spectrophotometry.An accurate fast spectrophotometric method for...

Age Estimation Based on Skin Surface Images

Xiang-qian HE, Ling-yu LI, Zheng JIANG, Hao LI
This paper presents a model of detection age which quantifies the appearance changes of human skin at different stages of aging without traditional skeleton. We provide a novel method to extract age-related primitive features from skin surface images obtained by a digital camera, including skin surface...

Analysis of Risk Factors for Uteroplacental Apoplexy Complicating Placental Abruption: A Systematic Review

Ning LIU, Yang LI, Yang CHEN, Yuan TIAN, Fu-ju WU
The paper explored risk factors for uteroplacental apoplexy complicating placental abruption, the condition's clinical characteristics, and maternal and fetal outcomes.62 pregnancies of placental abruption were collected from Second Hospital Jilin University during Jan. 2007-Dec. 2012.According to uteroplacental...

A Novel Indicator for Quantitative Assessment of Articular Cartilage Degeneration Using Ultrasound

Jing-chen ZHANG, Qiong HE, Zhi-xiu HAO, Jian-wen LUO, Li-ying XIAO
Assessment of articular cartilage degeneration induced by degenerative diseases using quantitative ultrasound method is promising but challenging. Currently, in literature, proposed methods have limited diagnostic abilities, and some quantitative ultrasound cartilage evaluation results are inconsistent...

Preparation on Sustained-Release Drug of Na-MMT-Miglitol

Jie-bing ZHANG, Hui SHI, Xiu-bin REN, Guang-heng WANG
In order to sucessfully prepare Na-MMT-Miglitol composite, the adsorption effect factors of the different concentrations, temperature , pH and time for miglitol were investigated respectively. By means of the adsorption capacity changes of Na-MMT, the maximum adsorption capacity of Na-MMT as the experimental...

A Micro/Nano Structural Model for Wettability of Moth Wing Surface

Yan FANG, Gang SUN, Jing-shi YIN, Wan-xing WANG, Yu-qian WANG
The micro-morphology, wettability and chemical composition of the moth wing surfaces were investigated by a scanning electron microscope (SEM), a contact angle (CA) meter and a Fourier transform infrared spectrometer (FT-IR). The micro-morphological models for hydrophobicity on the wing surface were...

Time-Dependent Cellular Injuries Induced by Calcium Oxalate Monohydrate and Dihydrate Crystals

Jian-Min WANG, Ling-Hong HUANG, Jian-Ming OUYANG
Objective: This study aims to compare the cytotoxicity and adhesion difference between oxalate monohydrate (COM) and dehydrate (COD) crystals and African green monkey kidney epithelial cells (Vero). Methods: Through the detection of cell viability, lactate dehydrogenase (LDH) release amount, expression...

Thinking of Toxicology in Chinese Medicine

Xu ZHANG, Yan-yan MIAO, Ming-san MIAO, Tian-yuan LIU, Tao ZHANG, Cai-ling FENG
This paper summarizes the research achievements of predecessors in the field of toxicology of Chinese medicine, discusses the new understanding of the toxicity of Chinese medicine from the aspects of the toxicity of Chinese medicine, the application of the toxicity of Chinese medicine and the trend of...

Valvular Heart Disease-Related Feed-Forward Loop Network Reveals Some Biological Features for Disease Development

Feng YANG, Ke LIU, Wei ZENG, Guang-bin WANG, Zheng-wen LI, Ke-li HUANG, Ni-ni RAO
This study is to uncover VHD-related important biological features for understanding molecular of this disease. The system analysis strategy is applied to generate the first VHD-related FFL network. Then, the network topology is mapped into the biological space to refine the biological features of VHD....

Visible Study of Mercuric Ion in Brassica Juncea Living Cells and Plants Using a Fluorescent Dye

Pin-san XU, Zheng-yao ZHANG, Ling-yao LI, Yu-bo LIU
Concern over mercury cytotoxicity inside live specimens has encouraged the development of efficient methods for the in vivo detection of this heavy metal. In this study, by using the previous developed fluorescence dye EPNP (2,6-bis-(4'-peperazino-N'-butyl-1',8'-naphthalimide) dimethylpyridine) as a...

Analyze the Animal Model of Pruritus Based on Clinical Symptoms of Chinese and Western Medicine

Ya-juan WEN, Ming BAI, Ming-san MIAO
Objective: Based on the characteristics of clinical symptoms of pruritus, analyze the relevant animal model, and then propose the evaluation and improvement. Method: According to the clinical diagnostic criteria of pruritus in Chinese and Western medicine, summarize the characteristics and modeling methods...

Isolation and Identification of a New Clostridium Butyricum XYB11 Strain Producing 1, 3-Propandiol from Soil

Yan-bo XUE, Miao-miao YANG, Jun-hua YUN, Tinashe Archbold MAGOCHA, Yi-lin QI, Lin-sen YANG, Ming-ming GUO, Fan Zou, Xiang-hui QI
1, 3-propanediol (1,3-PD) is an important chemical material, which is wildly used in many fields. Clostridium butyricumis key strain which can transform glycerol into 1,3-PD. In this study, a new strain producing 1,3-PD was isolated from soil. On the basis of morphology, biochemical-physiological characteristics,...

Effects of Tween 80 on Degradation of Benzo[ƒ]pyrene in Soils Growing Sudan Grass

Sheng-wang PAN, Zhi-hua LEI, Yun-xiao WU, Mao-ping HE
Phytoremediation is becoming a cost-effective technology for the in-situ clean up of sites polluted with hydrophobic organic contaminants (HOCs). The major factors limiting phytoremediation are the mass transfer, rate of plant uptake, and microbial biodegradation of HOCs. To evaluate the potential of...

Design and Development of Long Range Surface Plasmon Resonance Sensor Chip and System for High Sensitivity Detection

Lu-lu ZHANG, Xing CHEN, Cun-fang XU, Yan-fei REN, Ya-ting LI, Da-fu CUI
Long range surface plasmon resonance (LRSPR) was used to improve the propagation lengths, electric field strengths, and the sharpness of angular resonance curves compared with the conventional surface plasmon resonance (CSPR). LRSPR sensor chip was designed with buffer layer whose refractive indices...

Fed-Batch Fermentation of L- Threonine by Escherichia Coli Supplemented with B-Vitamins

Yue-wen SU, Qun-qun GUO, Sen WANG, Xin ZHANG, Jian WANG
B-vitamins can be used as cofactors in the synthesis process of L-threonine. Concentration of the cofactor can effectively change the activity of specific enzymes and reallocate the distribution of carbon substances in the cells. Theeffects of B-vitamins, including calcium pantothenate (VB5), cobalamin...

Exercise Related Sudden Death: The Changes of Expression of Bax, Bcl-2 Protain in Myocardial Tissue and Brain Motor Cortex

Yu QIAN, Ren-Jun YANG, Jin YIN
Purposes: Study the changes of cardiac and brain function of exercise related sudden death in rats under over fatigue state and explore the causes of sudden death preliminarily. Methods: Totally 72 male SD rats.7 rats were randomly selected as negative control group. The others were trained continuously...

Physiological and Biochemical Effect of Endurance Training of Altitude Training on Fixed Maximal Oxygen Expenditure in Middle and Long Distance Runners

Lang QIN, Jin YIN
The purpose of this study was to investigate the exercise intensity of endurance training of altitude training under the plain and plateau by means of the fixed maximal oxygen expenditure. Eight middle and long distance runners voluntarily participated in this study, the ergmeter bike and telemetry cardiopulmonary...

Preliminary Study on a RT-PCR Method for the Equine Infectious Anemia Virus Detection

Qin TANG, Han ZHANG, Jia-qi HAN, Li -rui LI, Ting-chuan LIU, Jing XU, Gang YU, Yin WANG, Xue-ping YAO, Ze-xiao YANG
To develop a Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method for quick detecting equine infectious anemia virus (EIAV), according the conserved sequences of EIAV gag gene which published in GenBank, 2 specific RT-PCR primers and 8 extension primers were designed and synthesized. A DNA...

One Orthogonal Phase Microscopy Technique for Orthogonal Phase Imaging of a Cell

Jing-ye LIU, Yuan-yuan XU, Qiong ZHU, Meng-yuan SHANG, Jing-rong LIAO, Ya-wei WANG
For human beings, the morphological features of cells are closely related to human health thus research into the technique of cell imaging is very important. The optical phase imaging technique is one of the most useful methods because the cell is a phase object. More than one phase images should be...

The Histological Study of Orthodontic Force on the Periodontal Tissues Regenerated by Nano Bioceramics in Beagle Dogs

Lan-lei WANG, He-ying HOU, Sheng-yan YU, Ai-zhong GUAN, Yun-mao LIAO
The aim of the experiment was to study the histological reaction of orthodontic force on the periodontal tissues regenerated by nano biphasic calcium phosphate (NBCP) bioceramics in beagle dogs. Two third incisors were randomly selected and operated as experimental group. The contralateral third incisors...

Miniaturized System with a Facile Isothermal Amplification Microfluidic Chip for Rapid Detection of Zika Viruses

Xing CHEN, Lu-lu ZHANG, Da-fu CUI, Hong YIN
A real-time fluorescence detection biomedical miniaturized system with a disposable microfluidic chip was developed for loop-mediated isothermal amplification (LAMP) reactions. The miniaturized system was developed consisting of a mini heating plate, a temperature sensor, a temperature controller and...