Preliminary Study on a RT-PCR Method for the Equine Infectious Anemia Virus Detection

DOI

10.2991/bbe-17.2017.51How to use a DOI?

Keywords

Equine infectious anemia, RT-PCR, Detection.

Abstract

To develop a Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method for quick detecting equine infectious anemia virus (EIAV), according the conserved sequences of EIAV gag gene which published in GenBank, 2 specific RT-PCR primers and 8 extension primers were designed and synthesized. A DNA fragment of EIAV gag gene about 370bp was synthesized in vitro by overlapping PCR, then the RT-PCR method for equine infectious anemia detection was established preliminarily through optimizing the reaction conditions, sensitivity and specificity experiments, and validity test. The results showed that the method for the EIAV has a good specificity and sensitivity, and the reaction product was about 370bp DNA electro phoretic band. This method can detect the EIAV target fragment about 119copies/ L. There are no amplification for the detection of Proteus, Actinobacillus, Salmonella, Escherichia coli, Stenotrophomonasmalt ophilia and Streptococcus type II, and the results showed positive reaction for Equine infectious anemia test antigen detection.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Qin TANG
AU  - Han ZHANG
AU  - Jia-qi HAN
AU  - Li -rui LI
AU  - Ting-chuan LIU
AU  - Jing XU
AU  - Gang YU
AU  - Yin WANG
AU  - Xue-ping YAO
AU  - Ze-xiao YANG
PY  - 2017/05
DA  - 2017/05
TI  - Preliminary Study on a RT-PCR Method for the Equine Infectious Anemia Virus Detection
BT  - Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SP  - 317
EP  - 324
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.51
DO  - 10.2991/bbe-17.2017.51
ID  - TANG2017/05
ER  -