Characterization of a Serine Carboxypeptidase Hoscp from Holotrichia Oblita Faldermann (Coleoptera: Scarabaeoidea)

DOI

10.2991/bbe-17.2017.21How to use a DOI?

Keywords

Holotrichia oblita, Serine carboxypeptidase, Gene cloning, Enzyme activity.

Abstract

In this report, we have identified a novel serine carboxypeptidase, named HoSCP, from the midgut of a destructive agricultural and landscape pest, Holotrichia oblita. HoSCP cDNA gene was cloned by immunoscreening method. Sequence analysis showed about 49kDa HoSCP consists of 457 amino acid residues with a 17-amino acid signal peptide and a conserved peptidase S10 family domain. The HoSCP protein was expressed as a recombinant protein in the yeast Pichia pastoris and enzyme activity assay showed that recombinant HoSCP had serine carboxypeptidases activity. Results of carboxypeptidase activities indicated that optimal enzyme activity occurs at pH 8.0, Compared with late 2nd-instar and late 3rd-instar larvae, midgut tissues of early 2nd-instar and early 3rd-instar larvae had higher levels of serine carboxypeptidase activity. The study would provide a foundation for the further research of the structure and function of HoSCP of H. oblita.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Dan ZHAO
AU  - Ya-kun ZHANG
AU  - Xiao-ping YAN
AU  - Wei GUO
AU  - Xiao-min LIU
PY  - 2017/05
DA  - 2017/05
TI  - Characterization of a Serine Carboxypeptidase Hoscp from Holotrichia Oblita Faldermann (Coleoptera: Scarabaeoidea)
BT  - Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SP  - 125
EP  - 133
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.21
DO  - 10.2991/bbe-17.2017.21
ID  - ZHAO2017/05
ER  -