Visible Study of Mercuric Ion in Brassica Juncea Living Cells and Plants Using a Fluorescent Dye
Pin-san XU, Zheng-yao ZHANG, Ling-yao LI, Yu-bo LIU
Available Online May 2017.
- https://doi.org/10.2991/bbe-17.2017.18How to use a DOI?
- Brassica juncea, Mercuric ion, Fluorescent dye.
- Concern over mercury cytotoxicity inside live specimens has encouraged the development of efficient methods for the in vivo detection of this heavy metal. In this study, by using the previous developed fluorescence dye EPNP (2,6-bis-(4'-peperazino-N'-butyl-1',8'-naphthalimide) dimethylpyridine) as a highly selective and sensitive probe for mercuric ions (Hg2+), the images of Hg2+ distribution and transportation in the living cells and plants of Brassica juncea were obtained. After 10, 50, 100 M Hg2+ exposure, gradually increased fluorescence was detected in B. juncea living cells loaded with EPNP dye. Intracellular Hg2+ time-dependent fluorescence enhancement of EPNP was also observed in B. juncea cells. Furthermore, images of confocal microscope shown that Hg2+ was found to mostly accumulate in lytic vacuole but not in the nucleus or mitochondria. In addition, a concentration-dependent rise of fluorescence was observed when the EPNP interacted with an increasing concentration of Hg2+ in the plants of B. juncea. These results provided direct experimental evidences for the distribution, translocation and transportation of Hg2+ in B. juncea. EPNP would be used as a chemical tool for the study of phytoremediation related researches.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Pin-san XU AU - Zheng-yao ZHANG AU - Ling-yao LI AU - Yu-bo LIU PY - 2017/05 DA - 2017/05 TI - Visible Study of Mercuric Ion in Brassica Juncea Living Cells and Plants Using a Fluorescent Dye BT - 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017) PB - Atlantis Press SN - 2468-5747 UR - https://doi.org/10.2991/bbe-17.2017.18 DO - https://doi.org/10.2991/bbe-17.2017.18 ID - XU2017/05 ER -