A Food-Grade, Secretory Expression of IL1RA Protein in a Lactococcus Lactis System for Potential Oral Therapy on IBD

DOI

10.2991/bbe-17.2017.61How to use a DOI?

Keywords

Interleukin 1 receptor antagonist (IL1RA), Lactococcus lactis, Recombinant, Inflammatory bowel disease (IBD).

Abstract

Interleukin 1 (IL1 ) is a potential target for IBD treatment. IL1 receptor antagonist (IL1RA) can blocks the biologic activity of IL1 by competitively inhibiting IL1 binding to the IL1R, so IL1RA is suggested to have possible therapeutic applications in treatment of IBD. By using a food-grade recombinant Lactococcus lactis system, a constitutive expression vector IL1RA:pNZ8149 was constructed and transferred into a L. lactis host NZ3900 in this study. The engineered strain was fermented and detected by colonies PCR and Western blot, the results indicate that the IL1RA secretory expression can be archived by IL1RA/NZ3900.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Jin CHENG
AU  - Jin CAO
AU  - Jia CAO
AU  - Xiao-feng WANG
AU  - Yan-ru GE
PY  - 2017/05
DA  - 2017/05
TI  - A Food-Grade, Secretory Expression of IL1RA Protein in a Lactococcus Lactis System for Potential Oral Therapy on IBD
BT  - Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SP  - 386
EP  - 391
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.61
DO  - 10.2991/bbe-17.2017.61
ID  - CHENG2017/05
ER  -