P4.19 THE EFFECT OF AN 8-WEEK, MODERATE-INTENSITY, AEROBIC EXERCISE INTERVENTION ON MMP-9 AND VASCULAR HAEMODYNAMICS

DOI

10.1016/j.artres.2013.10.137How to use a DOI?

Abstract

Objective: The Matrix Metalloproteinase-9 (MMP-9) enzyme is involved in degrading extracellular matrix (ECM) proteins, including collagen and elastin. Increased MMP-9 levels are associated with increased vascular remodelling and arterial stiffness. Exercise improves age-related vascular stiffening. It is therefore hypothesised that participation in an aerobic, moderate-intensity exercise programme will down-regulate mRNA expression of MMP-9.

Methods: Thirty-six healthy, sedentary individuals (43±14 yrs) joining an aerobic, moderate-intensity exercise programme, were recruited. Exercise (measured using IPAQ MET-minutes), supine blood pressure, augmentation index (AIx), aortic-pulse wave velocity (aPWV) and blood samples were obtained at baseline and 8-weeks into the programme. MMP-9 mRNA expression was analysed using real-time PCR, circulating MMP-9 levels assessed via ELISA, and AIx and aPWV were measured via applanation tonometry (SphygmoCor). Participants were split into those who adhered and did not adhere to the exercise programme.

Results: MET-minutes significantly increased; whilst MMP-9 expression, MAP and AIx significantly decreased in the exercise-adherent group compared to the non-adherent group, between baseline and 8-weeks. aPWV did not change significantly between the groups (Table-1). Significant correlations were observed between ΔIPAQ MET-minutes and ΔMMP-9 expression.

Conclusion: Adherence to the exercise programme reduced wave reflection and MAP. These pilot data demonstrate that MMP-9 expression is down-regulated in healthy, sedentary adults after 8-weeks of aerobic, moderate-intensity exercise. Thus suggesting that exercise-induced down-regulation of MMP-9 may result in a reduction in ECM degradation and therefore reduce vascular remodelling. These data provide a biomolecular rationale for how aerobic exercise is effective at delaying age-related increases in aPWV.

Parameter	Adherent n=17 (Mean±SE)	Non-Adherent n=19 (Mean±SE)	P Value
Δ IPAQ (MET-minutes)	+2848 ±547	−404 ±267	<0.001
Δ MMP-9 (Ct)	−0.56 ±0.09	+0.42 ±0.44	0.034
Δ Supine MAP (mmHg)	−2.64 ±1.59	+1.45 ±1.16	0.044
Δ Supine AIx@75 (%)	−4.1 ±1.75	+1.9 ±2.23	0.045
Δ CF-PWV (m/s)	+0.3 ±0.2	+0.2 ±0.2	0.854

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TY  - JOUR
AU  - J.E.S. Thompson
AU  - R. Webb
AU  - P. Hewlett
AU  - D. Llewellyn
AU  - B.J. McDonnell
PY  - 2013
DA  - 2013/11/11
TI  - P4.19 THE EFFECT OF AN 8-WEEK, MODERATE-INTENSITY, AEROBIC EXERCISE INTERVENTION ON MMP-9 AND VASCULAR HAEMODYNAMICS
JO  - Artery Research
SP  - 140
EP  - 141
VL  - 7
IS  - 3-4
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2013.10.137
DO  - 10.1016/j.artres.2013.10.137
ID  - Thompson2013
ER  -