Proceedings of the International Conference on Management, Computer and Education Informatization

One-pot Synthesis of Dihydroorotate by Multienzyme Polypeptide CAD

Authors
Jiawen Jiang, Lirui Sun, Limin Zhu, Jiaying Xin
Corresponding Author
Jiawen Jiang
Available Online June 2015.
DOI
https://doi.org/10.2991/mcei-15.2015.105How to use a DOI?
Keywords
Multienzyme polypeptide; CAD; Multi-step reaction; Dihydroorotate
Abstract
In the present research work, the multi-enzyme polypeptide carbamoyl synthetase-aspartate transcarbamoylase- dihydroorotase (CAD) was used as a novel biocatalyst to catalyze a multi-step reaction in a unitary reaction medium. Using organic solvent as reaction medium, we have observed formation of the final product, dihydroorotate, from initial substrates. Study on substrate specificity showed that the enzyme is capable of converting various amine to the corresponding dihydroortate (DHO). The multienzyme polypeptide CAD has an apparent molecular weight of 215,000-222,000.
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Proceedings
International Conference on Management, Computer and Education Informatization
Part of series
Advances in Computer Science Research
Publication Date
June 2015
ISBN
978-94-6252-118-6
ISSN
2352-538X
DOI
https://doi.org/10.2991/mcei-15.2015.105How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Jiawen Jiang
AU  - Lirui Sun
AU  - Limin Zhu
AU  - Jiaying Xin
PY  - 2015/06
DA  - 2015/06
TI  - One-pot Synthesis of Dihydroorotate by Multienzyme Polypeptide CAD
BT  - International Conference on Management, Computer and Education Informatization
PB  - Atlantis Press
SN  - 2352-538X
UR  - https://doi.org/10.2991/mcei-15.2015.105
DO  - https://doi.org/10.2991/mcei-15.2015.105
ID  - Jiang2015/06
ER  -