Proceedings of the International Symposium on Mechanical Engineering and Material Science

Study on Apoptosis of Human Hepatoma Cell line HepG-2 Induced by Total Alkaloids in H.littoralis

Authors
Ning Chen
Corresponding Author
Ning Chen
Available Online December 2016.
DOI
10.2991/ismems-16.2016.4How to use a DOI?
Keywords
Total Alkaloids in H.littoralis, Human Hepatoma Cell line HepG-2 Cells, Growth inhibition, apoptosis
Abstract

The purpose of this study is to investigate the effect of Total Alkaloids in H.littoralis on the induction of apoptosis involved in Human Hepatoma Cell line HepG-2. HepG-2 cells were treated with different concentrations of Alkaloids in H.littoralis at different time. MTT method was used to study Alkaloids in H.littoralis anti-tumor activity. Morphology observation was performed to determine the effects of Alkaloids in H.littoralis on apoptosis of HepG-2 cells. Flow cytometry (FCM) was used to detect the cell apoptosis rate and analyze the protein expression of Fas. The results showed Alkaloids in H.littoralis could inhibit the growth of HepG-2 cells, and the apoptosis morphology for 72h treatment was obvious. FCM indicated that the percentage of apoptotic cells increased with the raised concentration and the protein expression of Fas was increased after treated by Alkaloids in H.littoralis for 72h. In conclusion, Alkaloids of H.littoralis could induce HepG-2 cells apoptosis, and the mechanism may be related to death receptor pathwayThe purpose of this study is to investigate the effect of Total Alkaloids in H.littoralis on the induction of apoptosis involved in Human Hepatoma Cell line HepG-2. HepG-2 cells were treated with different concentrations of Alkaloids in H.littoralis at different time. MTT method was used to study Alkaloids in H.littoralis anti-tumor activity. Morphology observation was performed to determine the effects of Alkaloids in H.littoralis on apoptosis of HepG-2 cells. Flow cytometry (FCM) was used to detect the cell apoptosis rate and analyze the protein expression of Fas. The results showed Alkaloids in H.littoralis could inhibit the growth of HepG-2 cells, and the apoptosis morphology for 72h treatment was obvious. FCM indicated that the percentage of apoptotic cells increased with the raised concentration and the protein expression of Fas was increased after treated by Alkaloids in H.littoralis for 72h. In conclusion, Alkaloids of H.littoralis could induce HepG-2 cells apoptosis, and the mechanism may be related to death receptor pathwayThe purpose of this study is to investigate the effect of Total Alkaloids in H.littoralis on the induction of apoptosis involved in Human Hepatoma Cell line HepG-2. HepG-2 cells were treated with different concentrations of Alkaloids in H.littoralis at different time. MTT method was used to study Alkaloids in H.littoralis anti-tumor activity. Morphology observation was performed to determine the effects of Alkaloids in H.littoralis on apoptosis of HepG-2 cells. Flow cytometry (FCM) was used to detect the cell apoptosis rate and analyze the protein expression of Fas. The results showed Alkaloids in H.littoralis could inhibit the growth of HepG-2 cells, and the apoptosis morphology for 72h treatment was obvious. FCM indicated that the percentage of apoptotic cells increased with the raised concentration and the protein expression of Fas was increased after treated by Alkaloids in H.littorali

Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the International Symposium on Mechanical Engineering and Material Science
Series
Advances in Engineering Research
Publication Date
December 2016
ISBN
978-94-6252-277-0
ISSN
2352-5401
DOI
10.2991/ismems-16.2016.4How to use a DOI?
Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Ning Chen
PY  - 2016/12
DA  - 2016/12
TI  - Study on Apoptosis of Human Hepatoma Cell line HepG-2 Induced by Total Alkaloids in H.littoralis
BT  - Proceedings of the International Symposium on Mechanical Engineering and Material Science
PB  - Atlantis Press
SP  - 17
EP  - 21
SN  - 2352-5401
UR  - https://doi.org/10.2991/ismems-16.2016.4
DO  - 10.2991/ismems-16.2016.4
ID  - Chen2016/12
ER  -