Determination of carbapenem resistance mechanism in clinical isolates of Pseudomonas aeruginosa isolated from burn patients, in Tehran, Iran

Akbar Mirsalehian; Davood Kalantar-Neyestanaki; Morovat Taherikalani; Fereshteh Jabalameli; Mohammad Emaneini

doi:10.1016/j.jegh.2017.04.002

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Volume 7, Issue 3, September 2017, Pages 155 - 159

Determination of carbapenem resistance mechanism in clinical isolates of Pseudomonas aeruginosa isolated from burn patients, in Tehran, Iran

Authors

Akbar Mirsalehian^a, Davood Kalantar-Neyestanaki^b, Morovat Taherikalani^c, Fereshteh Jabalameli^a, Mohammad Emaneini^a^{, *}^,emaneini@tums.ac.ir

^aDepartment of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

^bStudent Research Committee, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

^cDepartment of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Lorestan, Iran

^*Corresponding author at: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, 100 Poursina St., Keshavarz Blvd., Tehran, Iran.

Corresponding Author

Mohammad Emaneiniemaneini@tums.ac.ir

Received 24 January 2016, Revised 12 March 2017, Accepted 22 April 2017, Available Online 29 April 2017.

DOI: 10.1016/j.jegh.2017.04.002 How to use a DOI?
Keywords: Pseudomonas aeruginosa; Carbapenem-resistant; Carbapenemase; AmpC; oprD
Abstract: Carbapenems are the most important therapeutic options that effect against serious infections caused by multidrug resistant Pseudomonas aeruginosa (MDR-PA) isolates. Carbapenems resistant isolates of P. aeruginosa are increasing worldwide. The aim of this study was to determine the carbapenem resistance mechanisms in clinical P. aeruginosa isolates from burn patients, in Tehran, Iran. A total of 53 non-duplicated isolates of carbapenem-resistant P. aeruginosa were collected from burn patients. The presence of carbapenemase genes were determined by PCR. AmpC overproducer isolates were detected by phenotypic method. The mutation and transcription level of oprD were determined by PCR-sequencing and quantitative Real-time PCR (RT-PCR), respectively. Twenty-seven (50.9%) isolates were positive for carbapenemase (bla_VIM = 25 and bla_IMP = 2) and showed high-level resistance to imipenem and meropenem. Twenty-eight isolates were AmpC overproducers. All isolates had a mutation in the oprD gene and down-regulation of oprD was found in 56.6% of MDR-PA isolates. Although the presence of carbapenemase is the common mechanism of resistant to carbapenem, but carbapenem resistance was found by oprD mutation-driven and the AmpC overproducing isolates in Tehran, Iran.
Open Access: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Journal: Journal of Epidemiology and Global Health
Volume-Issue: 7 - 3
Pages: 155 - 159
Publication Date: 2017/04/29
ISSN (Online): 2210-6014
ISSN (Print): 2210-6006
DOI: 10.1016/j.jegh.2017.04.002 How to use a DOI?
Open Access: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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ris enw bib

TY  - JOUR
AU  - Akbar Mirsalehian
AU  - Davood Kalantar-Neyestanaki
AU  - Morovat Taherikalani
AU  - Fereshteh Jabalameli
AU  - Mohammad Emaneini
PY  - 2017
DA  - 2017/04/29
TI  - Determination of carbapenem resistance mechanism in clinical isolates of Pseudomonas aeruginosa isolated from burn patients, in Tehran, Iran
JO  - Journal of Epidemiology and Global Health
SP  - 155
EP  - 159
VL  - 7
IS  - 3
SN  - 2210-6014
UR  - https://doi.org/10.1016/j.jegh.2017.04.002
DO  - 10.1016/j.jegh.2017.04.002
ID  - Mirsalehian2017
ER  -

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