Drug resistance detection and mutation patterns of multidrug resistant tuberculosis strains from children in Delhi

Jyoti Arora; Ritu Singhal; Manpreet Bhalla; Ajoy Verma; Niti Singh; Digamber Behera; Rohit Sarin; Vithal Prasad Myneedu

doi:10.1016/j.jegh.2016.12.003

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Volume 7, Issue 2, June 2017, Pages 141 - 145

Drug resistance detection and mutation patterns of multidrug resistant tuberculosis strains from children in Delhi

Authors

Jyoti Arora^a, Ritu Singhal^a, Manpreet Bhalla^a, Ajoy Verma^a, Niti Singh^a, Digamber Behera^b, Rohit Sarin^c, Vithal Prasad Myneedu^a^{, *}^,tbmicro@gmail.com

^aDepartment of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, Sri Aurobindo Marg, New Delhi, India

^bDepartment of Pulmonary Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India

^cDepartment of TB and Chest, National Institute of Tuberculosis and Respiratory Diseases, Sri Aurobindo Marg, New Delhi, India

^*Corresponding author at: Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, Sri Aurobindo Marg, New Delhi 110030, India.

Corresponding Author

Vithal Prasad Myneedutbmicro@gmail.com

Received 1 June 2016, Revised 26 November 2016, Accepted 15 December 2016, Available Online 7 February 2017.

DOI: 10.1016/j.jegh.2016.12.003 How to use a DOI?
Keywords: Drug resistance; Line probe assay; Retreatment cases; Smear positive; Tuberculosis
Abstract: A total of 312 sputum samples from pediatric patients presumptive of multidrug resistant tuberculosis were tested for the detection of drug resistance using the GenoTypeMTBDRplus assay. A total of 193 (61.8%) patients were smear positive and 119 (38.1%) were smear negative by Ziehl–Neelsen staining. Line probe assay (LPA) was performed for 208 samples/cultures (193 smear positive samples and 15 cultures from smear negative samples). Valid results were obtained from 198 tests. Of these, 125/198 (63.1%) were sensitive to both rifampicin (RIF) and isoniazid (INH). 73/198 (36.9%) were resistant to at least INH/RIF, out of which 49 (24.7%) were resistant to both INH and RIF (multidrug resistant). Children with tuberculosis are often infected by someone close to them, so strengthening of contact tracing in the program may help in early diagnosis to identify additional cases within the household. There is a need to evaluate newer diagnostic assays which have a high sensitivity in the case of smear negative samples, additional samples other than sputum among young children not able to expectorate, and also to fill the gap between estimated and reported cases under the program.
Open Access: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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<Previous Article In Issue

Journal: Journal of Epidemiology and Global Health
Volume-Issue: 7 - 2
Pages: 141 - 145
Publication Date: 2017/02/07
ISSN (Online): 2210-6014
ISSN (Print): 2210-6006
DOI: 10.1016/j.jegh.2016.12.003 How to use a DOI?
Open Access: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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ris enw bib

TY  - JOUR
AU  - Jyoti Arora
AU  - Ritu Singhal
AU  - Manpreet Bhalla
AU  - Ajoy Verma
AU  - Niti Singh
AU  - Digamber Behera
AU  - Rohit Sarin
AU  - Vithal Prasad Myneedu
PY  - 2017
DA  - 2017/02/07
TI  - Drug resistance detection and mutation patterns of multidrug resistant tuberculosis strains from children in Delhi
JO  - Journal of Epidemiology and Global Health
SP  - 141
EP  - 145
VL  - 7
IS  - 2
SN  - 2210-6014
UR  - https://doi.org/10.1016/j.jegh.2016.12.003
DO  - 10.1016/j.jegh.2016.12.003
ID  - Arora2017
ER  -

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