Proceedings of the Conference on Natural Resources And Life Sciences 2022 (NRLS-BIO 2022)

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Explant surface sterilization protocol for micropropagation of Amorphophallus muelleri Blume

Authors
Fenny Irawati1, Agnes Natalia Wijaya1, Anggi Manurung1, Michael Anthony Thongiratama1, Wina Dian Savitri1, Popy Hartatie Hardjo1, *
1Faculty of Biotechnology, University of Surabaya. Jl. Raya Kalirungkut, Surabaya, 60292, East Java, Indonesia
*Corresponding author. Email: poppy_hardjo@staff.ubaya.ac.id
Corresponding Author
Popy Hartatie Hardjo
Available Online 30 December 2023.
DOI
10.2991/978-94-6463-322-1_16How to use a DOI?
Keywords
aseptic technique; bulbil; sterilant agent; sterile explant
ABSTRACT

The success of tissue culture is greatly influenced by the explant surface sterilization technique. The presence of bacterial and fungi contamination, and the occurrence of browning on the explants can interfere with the process of culture propagation. High concentration of sterilant agents will inhibit cell division and the growth of explant tissues. This study aims to determine the optimum type and concentration of sterilant agents and how to use them to sterilize explants without causing much damage to explant tissue. The surface sterilization treatment of bulbil explant by soaking in a 250 mg.L-1 cefotaxime antibiotic solution for 30 min, and followed by immersion in 0.2% HgCl2 for 7 min, followed by soaking in NaOCl 1:2 for 10 min showed the best results obtained 60% sterile explants and faster growth of callus cells from explants. Callus grows to form adventitious buds within a period of 7 weeks.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the Conference on Natural Resources And Life Sciences 2022 (NRLS-BIO 2022)
Series
Advances in Biological Sciences Research
Publication Date
30 December 2023
ISBN
978-94-6463-322-1
ISSN
2468-5747
DOI
10.2991/978-94-6463-322-1_16How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Fenny Irawati
AU  - Agnes Natalia Wijaya
AU  - Anggi Manurung
AU  - Michael Anthony Thongiratama
AU  - Wina Dian Savitri
AU  - Popy Hartatie Hardjo
PY  - 2023
DA  - 2023/12/30
TI  - Explant surface sterilization protocol for micropropagation of Amorphophallus muelleri Blume
BT  - Proceedings of the Conference on Natural Resources And Life Sciences 2022 (NRLS-BIO 2022)
PB  - Atlantis Press
SP  - 116
EP  - 121
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-322-1_16
DO  - 10.2991/978-94-6463-322-1_16
ID  - Irawati2023
ER  -