Proceedings of the Conference on Natural Resources And Life Sciences 2022 (NRLS-BIO 2022)

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Utilization of Tempeh Extract as an Organic Supplement Alternative for Banana Tissue Culture

Authors
Alexander Willy Dimaswarabrata1, Anastasia Tatik Hartanti1, Listya Utami Karmawan1, *
1Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta, 12930, Indonesia
*Corresponding author. Email: listya.utami@atmajaya.ac.id
Corresponding Author
Listya Utami Karmawan
Available Online 30 December 2023.
DOI
10.2991/978-94-6463-322-1_17How to use a DOI?
Keywords
banana tissue culture; growth; medium; organic supplementation; tempeh
ABSTRACT

The addition of organic materials to tissue culture media has been known to have a positive impact on plant growth. However, a tissue culture medium utilizing organic supplements originating from Indonesia as its specialty, such as tempeh, has not been discovered. This study aims to determine the effect of adding tempeh extract to the tissue culture medium on the growth of Pisang Ambon Kuning. The media was supplemented with tempeh extract at concentrations of 5%, 15%, 25%, and 50% v/v. The banana plantlets used were 8 months old. In the initiation stage, explants inoculated in media supplemented with tempeh extract did not show any shoot growth. Similar results were shown in the subculture stage where the tempeh extract treatment demonstrated poor growth compared to the control treatment. Despite its potential to support growth, tempeh extract can hinder culture growth. The suboptimal growth results are suspected to be a stress response to the high copper content in tempeh extract, which is approximately 0.24 ± 0.02 mg.L−1.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the Conference on Natural Resources And Life Sciences 2022 (NRLS-BIO 2022)
Series
Advances in Biological Sciences Research
Publication Date
30 December 2023
ISBN
10.2991/978-94-6463-322-1_17
ISSN
2468-5747
DOI
10.2991/978-94-6463-322-1_17How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Alexander Willy Dimaswarabrata
AU  - Anastasia Tatik Hartanti
AU  - Listya Utami Karmawan
PY  - 2023
DA  - 2023/12/30
TI  - Utilization of Tempeh Extract as an Organic Supplement Alternative for Banana Tissue Culture
BT  - Proceedings of the Conference on Natural Resources And Life Sciences 2022 (NRLS-BIO 2022)
PB  - Atlantis Press
SP  - 122
EP  - 129
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-322-1_17
DO  - 10.2991/978-94-6463-322-1_17
ID  - Dimaswarabrata2023
ER  -