Cloning and Site-specific Mutagenesis of DS Enzyme Gene of Corynebacterium glutamicum
Authors
Hansong Yu, Shuang Qu, Yuhua Wang, Chunhong Piao, Junmei Liu, Yaohui Hu
Corresponding Author
Hansong Yu
Available Online April 2015.
- DOI
- 10.2991/mebe-15.2015.26How to use a DOI?
- Keywords
- Fermentation Engineering; Corynebacterium glutamicum; key enzyme of amino acid
- Abstract
In order to obtain the higher activity DS enzyme through genetic engineering technology. The point mutation method is used to get a higher activity recombinant DS enzyme from Corynebacterium glutamicum. At last the point 183 was mutated from L to Q in LG-322.The mutation type is a fundermental of construct a genetic engineering which has high activity DS enzyme.
- Copyright
- © 2015, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Hansong Yu AU - Shuang Qu AU - Yuhua Wang AU - Chunhong Piao AU - Junmei Liu AU - Yaohui Hu PY - 2015/04 DA - 2015/04 TI - Cloning and Site-specific Mutagenesis of DS Enzyme Gene of Corynebacterium glutamicum BT - Proceedings of the 2015 International Conference on Materials, Environmental and Biological Engineering PB - Atlantis Press SP - 106 EP - 110 SN - 2352-5401 UR - https://doi.org/10.2991/mebe-15.2015.26 DO - 10.2991/mebe-15.2015.26 ID - Yu2015/04 ER -