Analysis Gut Microbiota in Pregnant Women Trimester Three and Their Babies as Diagnostic Test of Degenerative Diseases

DOI

10.2991/978-94-6463-457-0_7How to use a DOI?

Keywords

Gut Microbiota; Metagenomics; Pregnant Women in Their Third Trimester

Abstract

This study aims to evaluate gut microbiota colonization in the first two years of a child’s life and explore the potential of the gut microbiota for the development of in vivo diagnostics (IVD) of degenerative diseases by reconstructing the dynamics of early colonization of gut microbiota at a genomic scale for the development of degenerative disease diagnostic kits. Data is being collected from pregnant ladies in their third trimester who will be used as research samples. This information comprises a health history, food (nutrition), and the way of delivering breast milk or formula milk for moms who will give birth to their babies, as well as the manner of giving birth, which is either normal or caesarean section. Interviews and questionnaires were used to acquire the sample data. Following the third stage of data processing from respondents, samples (whole blood, urine, and feces) were collected from healthy mothers and newborn babies. Whole blood, urine, and feces samples were obtained from pregnant women in their third trimester and newborns born in various locations, and each was labeled with the sample ID number. In the laboratory, samples were screened for Toxoplasma gondii microorganisms using the Rapid Diagnostic Test (RDT) to determine the presence of antibodies against T. gondii in serum and plasma samples (DALF Toxo-CA), and DNA was extracted and isolated from feces. Thus, amplifying the sample DNA with Polymerase Chain Reactions (PCR) and proceeding with sequencing.

Copyright

© 2024 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Nurisyah
AU  - Ratnasari Dewi
AU  - Mudyawati Kamaruddin
AU  - Miftahul Khairiyah Zamzam
AU  - Afra Anisah Purwadi
AU  - Abdur Rahman
AU  - Ana Hidayati Mukaromah
AU  - Andi Fatmawati
PY  - 2024
DA  - 2024/07/22
TI  - Analysis Gut Microbiota in Pregnant Women Trimester Three and Their Babies as Diagnostic Test of Degenerative Diseases
BT  - Proceedings of the 2nd Lawang Sewu International Symposium on Health Sciences: Medical Laboratory Technology (LSISHS-MLT 2023)
PB  - Atlantis Press
SP  - 54
EP  - 64
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-457-0_7
DO  - 10.2991/978-94-6463-457-0_7
ID  - 2024
ER  -