The Effect of Carbonate Hydroxyapatite (CHA) Dental Implant Material on the Early Development of Zebrafish Embryos (Danio rerio)

DOI

10.2991/absr.k.210621.052How to use a DOI?

Keywords

Biocompatibility, CHA, Dental, Embryo, Hatching, Zebrafish

Abstract

Carbonate Hydroxyapatite (CHA) is one of the bioceramic materials that can be used as dental implants. As dental implant material, the biocompatibility of CHA is an important prerequisite factor. This study aims to determine the biocompatibility of CHA on zebrafish embryos. The zebrafish embryos treatment was carried out on 3 hours post-fertilization (hpf) embryos aged. The embryos were exposed to CHA with concentrations of 15 µg/ml, 125 µg/ml, 500 µg/ml, and 2000 µg/ml, respectively. Biocompatibility assessment was carried out by measuring the hatching rate, survival, morphological changes, and embryo physiological performance, respectively. The hatching rate was determined as the number of embryos that hatched at 48, 72, and 96 hpf. The survival rate was determined by the number of live embryos at 24, 48, 72, and 96 hpf, respectively. Moreover, the morphological change was observed on the shape of the embryo’s heart and yolk, as well as the tail-yolk detachment as an indicator of a normal body wall occurrence, respectively. Moreover, the physiological performance was decided base on the heartbeat rate performance of the embryos. The result showed no effect of CHA on the hatching and survival rate as well as embryo morphology in all treatments. All embryos heart laterality was normally formed, and the tail-yolk detachment occurred at the period of 24 hpf, which indicated normal development of the embryo. The heartbeat rate showed no differences between CHA treatment compared to control, where the embryo heartbeat rate in all treatments was more than 200/minute, indicating normal embryos development. In conclusion, the CHA treatment did not cause any measurable development defect on zebrafish embryos.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Sandi Fransisco Pratama
AU  - Ika Dewi Ana
AU  - Bambang Retnoaji
PY  - 2021
DA  - 2021/06/23
TI  - The Effect of Carbonate Hydroxyapatite (CHA) Dental Implant Material on the Early Development of Zebrafish Embryos (Danio rerio)
BT  - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
PB  - Atlantis Press
SP  - 307
EP  - 312
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210621.052
DO  - 10.2991/absr.k.210621.052
ID  - Pratama2021
ER  -