Proceedings of the 9th International Seminar on Tropical Animal Production (ISTAP 2021)

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The Effect of Glutathione Addition in Diluent Semen on Ram Spermatozoa Quality

Authors
Muthiah Syafitri1, Teguh Ari Prabowo1, Pradita Iustitia Sitaresmi1, Lies Mira Yusiati2, Sigit Bintara1, Diah Tri Widayati1, *
1Department of Animal Breeding and Reproduction, Faculty of Animal Science, UGM, Indonesia
2Department of Animal Nutrition and Feed Science, Faculty of Animal Science, UGM, Indonesia
*Corresponding author. Email: widayati@ugm.ac.id
Corresponding Author
Diah Tri Widayati
Available Online 24 February 2022.
DOI
10.2991/absr.k.220207.052How to use a DOI?
Keywords
andromed diluent; antioxidant agent; motility; membrane integrity; ROS; semen preservation
Abstract

Cryopreservation of ram sperm reduces sperm motility and breaks sperm membrane integrity due to free radicals developed by reactive oxygen species (ROS). Moreover, it provokes cryodamage and deteriorates sperm quality. This study investigated the effect of glutathione addition as an antioxidant agent on the quality of local ram spermatozoa. The impacts of glutathione additions were observed in five different concentrations (control, 0.5 mM, 1 mM, and 1.5 mM or treatment P0-P4); the extender used to dilute the semen of two rams was andromed diluents. This research examined factors including the quality of fresh sperm, sperm motility, and membrane integrity after chilled. The data in this research was analyzed using one-way ANOVA to determine the effect of glutathione addition between the control and treatment groups. The outcomes demonstrated that adding glutathione significantly (p<0.05) enhanced motility and membrane integrity after stored in cold storage for 48 hours. In comparison with the control, the P1 concentration generated the best results in terms of ensuring the integrity of ram spermatozoa after processing. Glutathione concentration of 0.1mM (P1) showed the best motility and membrane integrity at 0 hours and 48 hours cold storage (82.50 ± 0.92 versus 65.35 ± 1.80% and 87.50 ± 0.50 versus 68.75 ± 1, 83%. In conclusion, the addition of glutathione affected the quality of local sheep spermatozoa after cooling. We obtain the best results of membrane motility and integrity at the glutathione level of 0.1 nM.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the 9th International Seminar on Tropical Animal Production (ISTAP 2021)
Series
Advances in Biological Sciences Research
Publication Date
24 February 2022
ISBN
978-94-6239-542-8
ISSN
2468-5747
DOI
10.2991/absr.k.220207.052How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

risenwbib
TY  - CONF
AU  - Muthiah Syafitri
AU  - Teguh Ari Prabowo
AU  - Pradita Iustitia Sitaresmi
AU  - Lies Mira Yusiati
AU  - Sigit Bintara
AU  - Diah Tri Widayati
PY  - 2022
DA  - 2022/02/24
TI  - The Effect of Glutathione Addition in Diluent Semen on Ram Spermatozoa Quality
BT  - Proceedings of the 9th International Seminar on Tropical Animal Production (ISTAP 2021)
PB  - Atlantis Press
SP  - 251
EP  - 255
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220207.052
DO  - 10.2991/absr.k.220207.052
ID  - Syafitri2022
ER  -