Proceedings of the International Conference on Innovation in Food Science, Culinary Art, and Fashion Technology (INNOFATEC 2025)

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In Silico Design and Bioinformatic Validation of LAMP Primers Targeting Porcine Cytochrome b for Halal Authentication

Authors
Mutia Devi Ariyana1, Firman Fajar Perdhana1, Raudatul Jannah2, Lalu Unsunnidhal1, *
1Food Science and Technology Study Program, Faculty of Food Technology and Agroindustry, University of Mataram, Mataram, 83125, West Nusa Tenggara, Indonesia
2Midwifery Study Program, INKES YARSI Mataram, Mataram, 83125, West Nusa Tenggara, Indonesia
*Corresponding author. Email: lalu.unsunnidhal@unram.ac.id
Corresponding Author
Lalu Unsunnidhal
Available Online 7 December 2025.
DOI
10.2991/978-94-6463-908-7_6How to use a DOI?
Keywords
Halal authentication; LAMP; Porcine cytochrome b
Abstract

This study aimed to design and validate qPCR and LAMP primers targeting the porcine mitochondrial cytochrome b gene (GenBank: AF034253.1) for rapid and specific halal authentication in processed meat products. Using Primer3Plus and the NEB LAMP Primer Design Tool, optimized primers were developed based on parameters including primer length (18–25 nt), melting temperature (55–65 ℃), GC content (36–60%), and minimal secondary structure. Structural analysis of the Cyt b protein using AlphaFold 3 confirmed its suitability as a specific biomarker. In silico PCR validation using UCSC tools showed that both qPCR and LAMP primers produced single, specific amplicons in Pig without cross-reactivity to Cow, Sheep and Chicken. The LAMP method, which operates under isothermal conditions and uses 5 primers targeting five distinct regions, demonstrated high sensitivity and specificity, making it ideal for point-of-need applications. These findings indicate that the designed primers are reliable molecular tools for ensuring the integrity of halal-certified food products.

Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the International Conference on Innovation in Food Science, Culinary Art, and Fashion Technology (INNOFATEC 2025)
Series
Advances in Social Science, Education and Humanities Research
Publication Date
7 December 2025
ISBN
978-94-6463-908-7
ISSN
2352-5398
DOI
10.2991/978-94-6463-908-7_6How to use a DOI?
Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Mutia Devi Ariyana
AU  - Firman Fajar Perdhana
AU  - Raudatul Jannah
AU  - Lalu Unsunnidhal
PY  - 2025
DA  - 2025/12/07
TI  - In Silico Design and Bioinformatic Validation of LAMP Primers Targeting Porcine Cytochrome b for Halal Authentication
BT  - Proceedings of the International Conference on Innovation in Food Science, Culinary Art, and Fashion Technology (INNOFATEC 2025)
PB  - Atlantis Press
SP  - 62
EP  - 76
SN  - 2352-5398
UR  - https://doi.org/10.2991/978-94-6463-908-7_6
DO  - 10.2991/978-94-6463-908-7_6
ID  - Ariyana2025
ER  -