Isolation and Characterization Bacillus sp. Producing Cellulase Enzymes from Hanura Mangrove

DOI

10.2991/aer.k.210603.004How to use a DOI?

Keywords

mangrove ecosystem, Bacterial isolation, cellulolytic bacteria, cellulolytic index

Abstract

In the mangrove ecosystem, there are cellulolytic bacteria that act to decompose cellulose in nature. This study aims to obtain cellulolytic bacteria from mangrove forests in Hanura village. Bacterial isolation was carried out using Sea Water Complete Agar (SWCA) media which added Carboxymethyl Cellulose 0.5% (CMC). Characterization of isolates included colony and cell morphology, pH and NaCl stress test, pathogenicity test, and metal influence test on cellulolytic activity. The results of the study obtained 38 bacterial isolates. 16 isolates among them were cellulolytic bacteria and were able to grow well on pH (7 and 10) and NaCl (0%, 3% and 6%) stress media. IBK3, ID2K1 and IA2K3 isolates are non-pathogenic bacteria with rod-positive Gram-positive properties. IBK3 had the highest cellulolytic index of7.36 and grew well on the addition of Fe, Al, Pb and Cu metals.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Sumardi
AU  - Salman Farisi
AU  - Christina N. Ekowati
AU  - Cahya I. Listiyorini
PY  - 2021
DA  - 2021/06/04
TI  - Isolation and Characterization Bacillus sp. Producing Cellulase Enzymes from Hanura Mangrove
BT  - Proceedings of the International Conference on Sustainable Biomass (ICSB 2019)
PB  - Atlantis Press
SP  - 21
EP  - 25
SN  - 2352-5401
UR  - https://doi.org/10.2991/aer.k.210603.004
DO  - 10.2991/aer.k.210603.004
ID  - 2021
ER  -