Factors Affecting Antibacterial and Antifungal Properties of Microemulsion Gel containing Tea Tree Oil

DOI

10.2991/ahsr.k.210115.101How to use a DOI?

Keywords

Microemulsion, Tea tree oil, antibacterial, antifungal

Abstract

Tea tree oil (Melaleuca alternifolia) has been acknowledged for its antibacterial and antifungal properties. Previous publications revealed that the tea tree oil has antibacterial activity against Propionibacterium acnes, which is the leading cause of acne, Staphylococcus aureus that caused various skin infections, and antifungal activity against Candida albicans. In this work, we aim to investigate the effect of microemulsion preparations in various gelling agents to their antibacterial and antifungal properties. Microemulsions have smaller droplets size than conventional emulsions; thus, we hypothesized that there would be a different effect on their inhibitory properties. The tea tree oil is formulated into a microemulsion and conventional emulsion, followed by incorporation into different gelling agents: Carbopol 940, Hydroxy-propyl methylcellulose, and sodium alginate. Physicochemical properties, such as pH, viscosity, and physical stability, had also been tested. The antibacterial and antifungal properties were tested using agar well diffusion methods. The microemulsion size was (26.23 + 0.15) nm, the pH of the final gel formulations was 4 to 7, and all formulations were physically stable under room temperature. The diameter of inhibition zones against bacteria and fungus was statistically analyzed using the multifactorial ANOVA method. The factors significantly affecting antibacterial and antifungal activities are the species of fungus and bacteria (p <0.001), gelling agents in formulations (p <0.05), and emulsions preparations (p <0.001). It was also interesting to note that these factors were significant on the activities tested (p <0.05).

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Raditya Weka Nugraheni
AU  - Uswatun Chasanah
AU  - Mohammad Dzulkifli Maulana
AU  - Miftakhur Ridha Ayyunaini
AU  - Umma Istiqomah Dwiputri
AU  - Rizki Ayu Firnanda
PY  - 2021
DA  - 2021/01/16
TI  - Factors Affecting Antibacterial and Antifungal Properties of Microemulsion Gel containing Tea Tree Oil
BT  - Proceedings of the 4th International Conference on Sustainable Innovation 2020–Health Science and Nursing (ICoSIHSN 2020)
PB  - Atlantis Press
SP  - 515
EP  - 519
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.210115.101
DO  - 10.2991/ahsr.k.210115.101
ID  - Nugraheni2021
ER  -