Efficient Protocol for Introducing Regeneration of Adventitious Buds from Hypocotyl Explants in Jatropha curcas L.

DOI

10.2991/icmse-16.2016.14How to use a DOI?

Keywords

Jatropha curcas; Hypocotyl explant; Regeneration; Rooting; Thidiazuron,.

Abstract

A new strategy of high concentration short duration treatment of Thidiazuron (TDZ) for enhancing adventitious buds formation from hypocotyl explants were attempted in Jatropha curcas. The best shoot buds induction (81.91%) and number of buds per explant (10.16) were obtained from hypocotyl explants treated with 20 mg/L TDZ solution for 20 min, followed by 30 days of culture on hormone-free MS medium. The elongated shoots initiated roots to become intact plantlets in rooting medium containing indole-3-butyric acid (IBA) and L-glutamine (Gln), and the results showed that the initiation and growth of roots were effectively stimulated with supplementing 16 mg/L Gln into the rooting medium, and having the best rooting rate (51.39%). After acclimatization, an intact plantlet could usually be gained at 60 days of culture by using the culture protocol described in this study.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Ying Liu
AU  - Su-Fang Niu
AU  - Jian-Nong Lu
AU  - Sen Chen
AU  - Ya-Fei Wang
AU  - Hong-Bo Zhu
AU  - Lin-Feng Li
AU  - Yu-Zhen Shi
AU  - Xue-Gui Yin
PY  - 2016/12
DA  - 2016/12
TI  - Efficient Protocol for Introducing Regeneration of Adventitious Buds from Hypocotyl Explants in Jatropha curcas L.
BT  - Proceedings of the 4th 2016 International Conference on Material Science and Engineering (ICMSE 2016)
PB  - Atlantis Press
SP  - 76
EP  - 84
SN  - 2352-5401
UR  - https://doi.org/10.2991/icmse-16.2016.14
DO  - 10.2991/icmse-16.2016.14
ID  - Liu2016/12
ER  -