Experimental Study on Method of Measuring Cytochrome P450 in Fish Liver subcellular fractions

DOI

10.2991/icmmbe-16.2016.122How to use a DOI?

Keywords

CYP450, fish, enzyme active

Abstract

To study the method of measuring cytochrome P450 (CYP450) in fish liver subcelluar fractions ( S9 and microsomes). Fish liver microsome was obtained by differential velocity centrifugation, the concentration of protein of liver microsome was measured by Bradford, and the activity of mice CYP450 was detected by spectrophotometer. The result showed that this method is easy and reliable, and has good reproducibility.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Mengnan Shen
AU  - Na Li
AU  - Ruohan Wu
PY  - 2016/09
DA  - 2016/09
TI  - Experimental Study on Method of Measuring Cytochrome P450 in Fish Liver subcellular fractions
BT  - Proceedings of the6th International Conference on Mechatronics, Materials, Biotechnology and Environment (ICMMBE 2016)
PB  - Atlantis Press
SP  - 663
EP  - 666
SN  - 2352-5401
UR  - https://doi.org/10.2991/icmmbe-16.2016.122
DO  - 10.2991/icmmbe-16.2016.122
ID  - Shen2016/09
ER  -