Determination of deoxynivalenol in grain sorghum by chemiluminescence enzyme immunoassay

DOI

10.2991/icmemtc-16.2016.19How to use a DOI?

Keywords

Deoxynivalenol; Chemiluminescence enzyme immunoassay; grain sorghum

Abstract

A chemiluminescence enzyme-linked immunosorbent assay (CLEIA) with enhanced chemiluminescent was developed for the detection of deoxynivalenol (DON) in grain sorghum. Assay conditions, including concentrations of antibody and enzyme conjugate, Na+ (NaCl), organic solvent (methyl alcohol) and pH were investigated. The optimized CLEIA allowed the DON detection in a linear working range of 0.588-26.783 ng mL-1 with the IC50 value of 3.968ng mL-1 and a limit of detection (LOD) of 0.311 ng mL-1. The CLEIA was one time more sensitive than the colorimetric ELISA by using the same antibody and HRP-conjugate. A series of spiked grain sorghum were detected by the method. The recovery rate ranged from 71.68% -96.56%, and the coefficient variation (CV) ranged from 5.93% -13%.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Zhenzhen Chen
AU  - Lixin Zhu
AU  - Renrong Liu
AU  - Wei Meng
AU  - Na Wu
AU  - Fanfan Yang
AU  - Kaihong Li
AU  - Yifan Lang
PY  - 2016/04
DA  - 2016/04
TI  - Determination of deoxynivalenol in grain sorghum by chemiluminescence enzyme immunoassay
BT  - Proceedings of the 2016 3rd International Conference on Materials Engineering, Manufacturing Technology and Control
PB  - Atlantis Press
SP  - 102
EP  - 105
SN  - 2352-5401
UR  - https://doi.org/10.2991/icmemtc-16.2016.19
DO  - 10.2991/icmemtc-16.2016.19
ID  - Chen2016/04
ER  -