Proceedings of the 2016 3rd International Conference on Materials Engineering, Manufacturing Technology and Control

Identification of Vibrio parahaemolyticus Strains by means of HPLC fingerprinting

Authors
Yiling Tian, Yunzhe Zhang, Hui Shi, Wei Zhang
Corresponding Author
Yiling Tian
Available Online April 2016.
DOI
10.2991/icmemtc-16.2016.18How to use a DOI?
Keywords
Vibrio parahaemolyticus; identification; high performance liquid chromatography
Abstract

The objective of this study was to identify Vibrio parahaemolyticus by HPLC, a simple, rapid, and reliable HPLC method. For the HPLC methods, an established for the fingerprints of Vibrio parahaemolyticus was performed on Strict extraction and reversed-phase HPLC conditions. An Agilent C18(4. 6 mm ×25 mm, 5 m) as column and acetonitrile-0.05% phosphoric acid solution in gradient as mobile phase. The detection wavelength was set at 280 nm and column temperature was 30°C. Established HPLC fingerprint was distinct and reproducible. There were 2 characteristic peaks. The HPLC fingerprints of Vibrio parahaemolyticus is characteristic and specific, and has the potential to replace biochemical methods because of its simplicity, rapidity, specificity, and cost-effectiveness. It is apparent from this study that we had developed a new, steady and rapid method for identification of Vibrio parahaemolyticus Strains. For the rapid identification of foodborne pathogens build a technology platform.

Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2016 3rd International Conference on Materials Engineering, Manufacturing Technology and Control
Series
Advances in Engineering Research
Publication Date
April 2016
ISBN
978-94-6252-173-5
ISSN
2352-5401
DOI
10.2991/icmemtc-16.2016.18How to use a DOI?
Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Yiling Tian
AU  - Yunzhe Zhang
AU  - Hui Shi
AU  - Wei Zhang
PY  - 2016/04
DA  - 2016/04
TI  - Identification of Vibrio parahaemolyticus Strains by means of HPLC fingerprinting
BT  - Proceedings of the 2016 3rd International Conference on Materials Engineering, Manufacturing Technology and Control
PB  - Atlantis Press
SP  - 96
EP  - 101
SN  - 2352-5401
UR  - https://doi.org/10.2991/icmemtc-16.2016.18
DO  - 10.2991/icmemtc-16.2016.18
ID  - Tian2016/04
ER  -