Development of a chemiluminescence enzyme immunoassay for the detection of aflatoxin M1
Authors
Yifan Lang, Zhenzhen Chen, Lixin Zhu, Renrong Liu, Xuemei Qiu, Kaihong Li, Fanfan Yang, Na Hu
Corresponding Author
Yifan Lang
Available Online April 2016.
- DOI
- 10.2991/icmemtc-16.2016.20How to use a DOI?
- Keywords
- aflatoxin M1; chemiluminescence enzyme immunoassay
- Abstract
An indirect competitive chemiluminescence enzyme immunoassay (CLEIA) for detecting aflatoxin M1 (AFM1) was developed. In the experimentation, antigen coated concentration, antibody dilution proportion, pH value and ionic concentration of the buffer were optimized to obtain the best reaction condition. Finally, the 50% inhibitory concentration (IC50) of the optimized CLEIA is 0.11ng/mL for skimmed milk powder, and the recovery ranged from 77.98% to 104.39%.
- Copyright
- © 2016, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Yifan Lang AU - Zhenzhen Chen AU - Lixin Zhu AU - Renrong Liu AU - Xuemei Qiu AU - Kaihong Li AU - Fanfan Yang AU - Na Hu PY - 2016/04 DA - 2016/04 TI - Development of a chemiluminescence enzyme immunoassay for the detection of aflatoxin M1 BT - Proceedings of the 2016 3rd International Conference on Materials Engineering, Manufacturing Technology and Control PB - Atlantis Press SP - 106 EP - 109 SN - 2352-5401 UR - https://doi.org/10.2991/icmemtc-16.2016.20 DO - 10.2991/icmemtc-16.2016.20 ID - Lang2016/04 ER -