Proceedings of the 4th Annual International Conference on Material Engineering and Application (ICMEA 2017)

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Effect of Grafted Chain Length on Hemostatic Activity of N-alkylated Chitosan

Authors
Mengyuan Liu, Jian Yang, Jing Guan, Shujie Huang, Zhihong Li, Miaolei Jing
Corresponding Author
Mengyuan Liu
Available Online February 2018.
DOI
10.2991/icmea-17.2018.53How to use a DOI?
Keywords
chitosan; alkylation; blood coagulation
Abstract

This study aimed to synthesize N-alkylated chitosan derivates with similar degree of substitution (DS) and alkyl groups of different carbon chain lengths via reductive alkylation, and then to compare their coagulation properties. Structural properties of the N-alkylated chitosan derivates were characterized by Fourier transform infrared spectroscopy, elemental analysis and 1H nuclear magnetic resonance spectroscopy. Their pro-coagulant properties were evaluated by detecting whole blood clotting time and performing TEG test. Pro-coagulant mechanisms of the N-alkylated chitosan derivates were explored by detecting intracellular Ca2+ concentration in platelets and P-selectin expression on platelets. We successfully prepared N-alkylated chitosan derivates with a DS of 32.88%-39.78%, including NACS6, NACS12 and NACS18. Our experimental findings showed that all N-alkylated chitosan derivates (NACS6, NACS12, NACS18) have outstanding procoagulant effects, and the longer alkyl chain, the better procoagulant ability. However, N-alkylated chitosan cannot activate platelets, and its procoagulant mechanism remains to be further studied in future. Based on the above results, this paper makes a conjecture about the coagulation mechanism of N-alkylation chitosan.

Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 4th Annual International Conference on Material Engineering and Application (ICMEA 2017)
Series
Advances in Engineering Research
Publication Date
February 2018
ISBN
10.2991/icmea-17.2018.53
ISSN
2352-5401
DOI
10.2991/icmea-17.2018.53How to use a DOI?
Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Mengyuan Liu
AU  - Jian Yang
AU  - Jing Guan
AU  - Shujie Huang
AU  - Zhihong Li
AU  - Miaolei Jing
PY  - 2018/02
DA  - 2018/02
TI  - Effect of Grafted Chain Length on Hemostatic Activity of N-alkylated Chitosan
BT  - Proceedings of the 4th Annual International Conference on Material Engineering and Application (ICMEA 2017)
PB  - Atlantis Press
SP  - 229
EP  - 234
SN  - 2352-5401
UR  - https://doi.org/10.2991/icmea-17.2018.53
DO  - 10.2991/icmea-17.2018.53
ID  - Liu2018/02
ER  -