Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

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Evaluation of Antibacterial and Antioxidant Activities of Stingless Bee (Tetragonula Laeviceps) Hive from Purwodadi Botanical Garden and LC/MS Profiles of Dichloromethane Fraction

Authors
Praptiwi Praptiwi1, *, Ahmad Fathoni1, Oscar Efendy1, Dewi Wulansari1, Andria Agusta1
1Research Center for Pharmaceutical Ingredients and Traditional Medicine, The National Research and Innovation Agency (BRIN), Bogor, Indonesia
*Corresponding author. Email: praptiwip@yahoo.com
Corresponding Author
Praptiwi Praptiwi
Available Online 1 March 2023.
DOI
10.2991/978-94-6463-112-8_33How to use a DOI?
Keywords
Stingless bee; antibacterial; antioxidant
Abstract

This study evaluates the potential of stingless bee (Tetragonula laeviceps) methanol extract and its fractions (hexane, dichloromethane, ethyl acetate, methanol, and acetone-water) as an antibacterial, antioxidant, phenolics content, and LC/MS profiles of dichloromethane fraction. The test was carried out by thin-layer chromatography (TLC) - bioautography (dot-blot and elution), then determined the MIC and IC50 values and Antioxidant Activity Index (AAI). Antibacterial tests were carried out against Staphylococcus aureus and Escherichia coli, and antioxidant potential was carried out by the DPPH method. Total phenolic content was evaluated by the spectrophotometry method. The results showed that crude fraction, hexane, and dichloromethane fractions had moderate antibacterial activity against S. aureus (MIC values: 128–256 µg/mL) and weak antibacterial activity against E. coli. Fractions of dichloromethane, methanol, and acetone water had a very strong antioxidant activity with an AAI of 5.28, 2.17, 3.00, and 3.56, respectively. The LC/MS profiles showed that the dichloromethane fraction contained 5.7-dihydroxychromone, cnidimon C, puerarin, and irisflorentin. Fractions of dichloromethane, methanol, and acetone-water fraction are potential antioxidant and antibacterial sources.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
Series
Advances in Health Sciences Research
Publication Date
1 March 2023
ISBN
978-94-6463-112-8
ISSN
2468-5739
DOI
10.2991/978-94-6463-112-8_33How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Praptiwi Praptiwi
AU  - Ahmad Fathoni
AU  - Oscar Efendy
AU  - Dewi Wulansari
AU  - Andria Agusta
PY  - 2023
DA  - 2023/03/01
TI  - Evaluation of Antibacterial and Antioxidant Activities of Stingless Bee (Tetragonula Laeviceps) Hive from Purwodadi Botanical Garden and LC/MS Profiles of Dichloromethane Fraction
BT  - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
PB  - Atlantis Press
SP  - 349
EP  - 359
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-112-8_33
DO  - 10.2991/978-94-6463-112-8_33
ID  - Praptiwi2023
ER  -