Antioxidant, Antibacterial and Antibiofilm Activities of an Endophytic Fungi, Penicillium Sp. SAF6-EGY Strain AFL.2 and Arthrinium Sp. R22-1 Strain AFL.3 Isolated from Aglaia Foveolata

DOI

10.2991/978-94-6463-112-8_32How to use a DOI?

Keywords

Aglaia foveolate; Antibacterial; Arthrinium sp.; Endophytic fungi; Penicillium sp.

Abstract

Endophytic fungi are widely underexplored in the discovery of novel bioactive compounds with promising pharmaceutical uses. Aglaia foveolata, which has important medicinal value, was investigated for its endophytic fungi to produce bioactive compounds by determining antioxidant, antibacterial, and antibiofilm activities. In this study, two endophytic fungi were collected from A. foveolata leaves and identified phenotypically along with genotypically by Internal Transcribed Spacer (ITS) gene sequence and known as Penicillium sp. SAF6-EGY strain AFL.2 and Arthrinium sp. R22-1 strain AFL.3. These fungal cultures were further grown on Yeast Malt (YM) medium and the culture was extracted by ethyl acetate. The ethyl acetate extract of AFL.2 and AFL.3 showed relatively similar in Total Phenolic Content (TPC) and Total Flavonoid Content (TFC) ranging from 114.71 to 119.42 mg GAE/g extract and 74.09 until 76.31 mg QE/g extract for TPC and TFC, respectively. Further, AFL.3 showed the highest antioxidant activity in 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) scavenging at IC₅₀ value of 123.41 and 256.95 µg/mL, respectively. As for antibacterial activity, AFL.2 exhibited the strongest activity against Eschericia coli (acrB JW0451-2) and Staphylococcus aureus (Newman) with the same MIC value of 250 µg/mL. Meanwhile, AFL.3 showed the best antibacterial activity against Bacillus subtilis (DSM10) and S. aureus with the same MIC value of 500 µg/mL. In addition, AFL.2 showed the best antibiofilm activity against E. coli, while AFL.3 against B. subtilis biofilm with the inhibition value of 42 and 35%, respectively. Liquid Chromatography Quadrupole-Mass Spectrometry Analysis (LC-MS/MS) revealed that both AFL.2 and AFL.3 extract contained several putative antibacterial compounds which are correspond to its promising activity.

Copyright

© 2023 The Author(s)

Open Access

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TY  - CONF
AU  - Muhammad E. Prastya
AU  - Jepri A. Priyanto
AU  - Gian Primahana
AU  - Tjandrawati Mozef
AU  - Abdi W. Septama
AU  - Rizna T. Dewi
AU  - Zetryana P. Tachrim
AU  - Agustina S. Andreani
AU  - Siti I. Rahmawati
AU  - Eris Septiana
PY  - 2023
DA  - 2023/03/01
TI  - Antioxidant, Antibacterial and Antibiofilm Activities of an Endophytic Fungi, Penicillium Sp. SAF6-EGY Strain AFL.2 and Arthrinium Sp. R22-1 Strain AFL.3 Isolated from Aglaia Foveolata
BT  - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
PB  - Atlantis Press
SP  - 338
EP  - 348
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-112-8_32
DO  - 10.2991/978-94-6463-112-8_32
ID  - Prastya2023
ER  -