Proceedings of the 4th International Conference on Halal Development (4th ICHaD 2023)

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Optimization of NaOH Pretreatment Time on Collagen Production from Milkfish Scales

Authors
Evi Susanti1, *, Syafira Maharani1, 2, Salsabila Garin Ramadanti2
1Biotechnology Program, Department of Applied Science, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang No. 5 Malang, Malang, East Java, 65145, Indonesia
2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang No. 5 Malang, Malang, East Java, 65145, Indonesia
*Corresponding author. Email: evi.susanti.fmipa@um.ac.id
Corresponding Author
Evi Susanti
Available Online 21 June 2024.
DOI
10.2991/978-2-38476-261-3_15How to use a DOI?
Keywords
collagen; pretreatment time; milkfish scales; protease
Abstract

The pretreatment using 0.1 M NaOH is widely used on fish scales in the collagen production stage. This process is necessary to remove non-collagen proteins, fats, and other impurities, but improper pretreatment can reduce colla- gen levels obtained because prolonged exposure will damage connective tissue on scales, resulting in decreased yield. This research aims to determine the opti- mum NaOH pretreatment time for the collagen yield produced and identify changes in milkfish scales chemical composition and surface morphology. The stages in this study are: 1) preparation of milkfish scales; 2) pretreatment of milk- fish scales with NaOH 0.1 M at various time variations of 0, 1, 3, 6, and 8 h; and 3) determination of the effect of pretreatment time on collagen yield pro- duced from milkfish scales using proteases from Bacillus megaterium TR-10. The determination of optimum conditions is based on the content of water, ash, fat, protein, metal ion content (Hg2+, Pb2+, K+), and surface morphology of milk- fish scales before and after NaOH pretreatment is carried out. The results showed that the highest yield of 56.77 ppm was achieved at the pretreatment treatment of NaOH for 6 h. The longer the NaOH pretreatment time, the water, ash, fat, protein, and metal content in the scales decreases, and the surface morphology of milkfish scales is cleaner, but in the 8-h treatment, damage to connective tis- sue fibers in fish scales is observed. The optimal NaOH pre-treatment time for collagen production from milkfish scales is 6 h of treatment.

Copyright
© 2024 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 4th International Conference on Halal Development (4th ICHaD 2023)
Series
Advances in Social Science, Education and Humanities Research
Publication Date
21 June 2024
ISBN
978-2-38476-261-3
ISSN
2352-5398
DOI
10.2991/978-2-38476-261-3_15How to use a DOI?
Copyright
© 2024 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Evi Susanti
AU  - Syafira Maharani
AU  - Salsabila Garin Ramadanti
PY  - 2024
DA  - 2024/06/21
TI  - Optimization of NaOH Pretreatment Time on Collagen Production from Milkfish Scales
BT  - Proceedings of the 4th International Conference on Halal Development (4th ICHaD 2023)
PB  - Atlantis Press
SP  - 162
EP  - 172
SN  - 2352-5398
UR  - https://doi.org/10.2991/978-2-38476-261-3_15
DO  - 10.2991/978-2-38476-261-3_15
ID  - Susanti2024
ER  -