The Immunomodulatory Activities of Alkaloid (Vf-1) Isolated from Stem Bark of Tampa Badak (Voacanga Foetida (Bl) Rolfe) on Raw 264.7 Cells

DOI

10.2991/ahsr.k.211105.039How to use a DOI?

Keywords

Voacanga foetida; immunomodulatory; IL-6; macrophages; LPS

Abstract

Voacanga foetida (Bl) Rolfe (Apocynaceae) locally called Tampa Badak is a widely distributed plant in Indonesia. Indonesian people use this plant as a traditional medicine to treat infections, pain, diarrhea, itching, and swelling. VF-1 is an alkaloid compound that has been previously isolated from the dichloromethane fraction of the stem bark of the Tampa Badak. Based on the melting point value and interpretation of the ¹H-NMR spectrum, it was described that the VF-1 alkaloid compound was similar to voacangine. This study was conducted to determine the immunomodulatory activity of VF-1 compounds from the stem bark of the Tampa Badak. Cell viability and immunomodulatory activity of the compounds were carried on cultured RAW 264.7 cells (macrophages). The effect of VF-1 compound on cell viability was carried out using the MTT method to determine the safety or toxicity of the compound against macrophage cells. The immunomodulatory activity of VF-1 was tested on lipopolysaccharide (LPS)-induced RAW 264.7 cells using the sandwich ELISA method by measuring the production of proinflammatory cytokines IL-6 in cell supernatants. The results showed that RAW 264.7 cells treated with VF-1 at concentrations of 1, 10, and 100 g/mL appeared safe and non-toxic and showing cell viability values >80%. The percentage inhibition of IL-6 production at each concentration of VF-1 was 45.85; 58.20; 23.25% respectively. Based on the results obtained, it was concluded that the compound has the potential as an immunosuppressant in RAW 264.7 macrophage cells induced by LPS.

Copyright

© 2021 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article under the CC BY-NC license.

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TY  - CONF
AU  - Zuriva Azwari
AU  - Dian Ayu Juwita
AU  - Adriani Susanty
AU  - Nurdina Putri
AU  - Dira Hefni
AU  - Dachriyanus
AU  - Fatma Sri Wahyuni
PY  - 2021
DA  - 2021/11/17
TI  - The Immunomodulatory Activities of Alkaloid (Vf-1) Isolated from Stem Bark of Tampa Badak (Voacanga Foetida (Bl) Rolfe) on Raw 264.7 Cells
BT  - Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021)
PB  - Atlantis Press
SP  - 268
EP  - 276
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.211105.039
DO  - 10.2991/ahsr.k.211105.039
ID  - Azwari2021
ER  -