Proceedings of the 2016 5th International Conference on Civil, Architectural and Hydraulic Engineering (ICCAHE 2016)

Characterization and Structural Identification of an 11S Globulin of Juglans mandshurica Maxim. Kernel from the Changbai Mountains in China

Authors
Li Fang, Xiaofei Wang, Chunlei Liu, Peng Wang, Wei Liu, Weihong Min
Corresponding Author
Li Fang
Available Online October 2016.
DOI
10.2991/iccahe-16.2016.89How to use a DOI?
Keywords
Juglans mandshurica Maxim.; Walnut; 11S globulin; purification; structural characterization
Abstract

The major seed storage protein 11S globulin from Juglans mandshurica Maxim. (JSG) was isolated and purified to identify its structure and analyze its physicochemical properties. JSG was found to be composed of two distinct major polypeptides with molecular masses of 23 and 20 kDa, and whose amino acid sequences are highly similar to those of the 11S globulin from J. regia in the NCBI database. JSG contains essential amino acids that correspond to the adult standard recommended by FAO/WTO. Differential scanning calorimetry revealed a single endothermic peak with a denaturation temperature of 96.83 øC and an enthalpy value of 12.07 J/g. The secondary structure of the JSG consists of -sheets (32.83%) and -helices (11.67%). A red shift was also observed in the fluorescence spectra with guanidine hydrochloride (GdnHCl) or urea as denaturant; GdnHCl was proven to be a stronger denaturant than urea.

Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2016 5th International Conference on Civil, Architectural and Hydraulic Engineering (ICCAHE 2016)
Series
Advances in Engineering Research
Publication Date
October 2016
ISBN
978-94-6252-250-3
ISSN
2352-5401
DOI
10.2991/iccahe-16.2016.89How to use a DOI?
Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Li Fang
AU  - Xiaofei Wang
AU  - Chunlei Liu
AU  - Peng Wang
AU  - Wei Liu
AU  - Weihong Min
PY  - 2016/10
DA  - 2016/10
TI  - Characterization and Structural Identification of an 11S Globulin of Juglans mandshurica Maxim. Kernel from the Changbai Mountains in China
BT  - Proceedings of the 2016 5th International Conference on Civil, Architectural and Hydraulic Engineering (ICCAHE 2016)
PB  - Atlantis Press
SP  - 533
EP  - 543
SN  - 2352-5401
UR  - https://doi.org/10.2991/iccahe-16.2016.89
DO  - 10.2991/iccahe-16.2016.89
ID  - Fang2016/10
ER  -