Sustainable Biogenic Synthesis of Silver Nanoparticles (AgNPs) using Aegle marmelos and Evaluation of their Photocatalytic, Antimicrobial and Cytotoxicity Properties

DOI

10.2991/978-94-6463-648-2_15How to use a DOI?

Keywords

Nanotechnology; Silver Nanoparticles; Aegle marmelos; cytotoxicity

Abstract

Nanotechnology has revolutionized materials research, leveraging nanoscale entities (1–100 nm) with extensive applications across diverse fields. This study explores the synthesis of silver nanoparticles (AgNPs) using aqueous leaf extract of Aegle marmelos (AMLAE) prepared via the decoction method. The Ag⁺ ions were reduced to Ag⁰ using AMLAE and without using any chemical for reduction and precipitation. The formation of AM-AgNPs was confirmed through UV-Vis spectroscopy, which exhibited a localized surface plasmon resonance (LSPR) band at 450 nm. Fourier-transform infrared (FTIR) analysis revealed surface functionalization, including O-H, C=C, C=O, and Ag-O bonds. X-ray diffraction (XRD) confirmed a face-centered cubic structure with an average crystallite size of 14.07 nm, while high-resolution transmission electron microscopy (HRTEM) indicated spherical morphology and an average particle size of 16.07 nm. Dynamic light scattering (DLS) analysis showed a hydrodynamic size of 237.5 nm with a polydispersity index (PDI) of 0.243 and a zeta potential of -16.6 mV, indicating colloidal stability. The antimicrobial efficacy of AM-AgNPs was assessed against six pathogens, with minimum inhibitory concentrations (MIC) as low as 15.62 μg mL⁻¹ for Vibrio parahaemolyticus. Photocatalytic degradation of methylene blue (50 ppm) demonstrated a 99.24% degradation efficiency under visible light within 60 minutes. Cytotoxicity studies on Vero cell lines yielded an IC50 value of 253.73 μg mL⁻¹, suggesting low toxicity levels. These findings represent the potential of green-synthesized AgNPs for biomedical and environmental applications, establishing AMLAE as an efficient and sustainable reducing agent.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Abhinash Marukurti
AU  - Alavala Matta Reddy
AU  - V. Durga Praveena
AU  - G. Tejaswini
AU  - M. Padmaja
AU  - L. Rajeswari
AU  - D. Sailaja
AU  - P. Vijaya Nirmala
AU  - N. Baby Nirmala
AU  - J. Shashi Sri
PY  - 2025
DA  - 2025/02/07
TI  - Sustainable Biogenic Synthesis of Silver Nanoparticles (AgNPs) using Aegle marmelos and Evaluation of their Photocatalytic, Antimicrobial and Cytotoxicity Properties
BT  - Proceedings of the International Conference on Bio-Based Environment for Sustainable Territory (ICBEST 2024)
PB  - Atlantis Press
SP  - 178
EP  - 194
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-648-2_15
DO  - 10.2991/978-94-6463-648-2_15
ID  - Marukurti2025
ER  -