Proceedings of the 6th International Conference of Food, Agriculture, and Natural Resource (IC-FANRES 2021)

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Prebiotic Potential of Oligosaccharides: In Vitro Study of Indonesian Local Honey from Apis spp. and Trigona spp. Bees

Authors
Fitria Susilowati1, *, Mita Nurul Azkia2
1Program Studi Gizi, Universitas Islam Negeri Walisongo, Semarang, 50185, Indonesia
2Program Studi Teknologi Hasil Pertanian, Universitas Semarang, Semarang, 50196, Indonesia
*Corresponding author. Email: fitria_susilowati@walisongo.ac.id
Corresponding Author
Fitria Susilowati
Available Online 5 January 2022.
DOI
10.2991/absr.k.220101.025How to use a DOI?
Keywords
Oligosaccharides; Prebiotic; Honey; Apis spp.; Trigona spp.
Abstract

Oligosaccharide compounds (FOS, GOS) have gained huge commercial interest due to their beneficial properties in human health as prebiotics. This study aims to isolate the oligosaccharides compounds and investigate the prebiotic potential of Indonesian local honey from Apis spp. (KR) and Trigona spp. (KL) honey bees. The isolation of oligosaccharides was done utilizing the adsorption activity of activated carbon. The qualitative assay to investigate the oligosaccharides was performed by TLC (Thin Layer Chromatography) method. The isolated oligosaccharides was determined quantitatively by the Phenol-Sulphuric Acid method (Total Sugar) and dinitrosalicylic acid (DNS) methods (Reducing Sugar). The total sugar content from oligosaccharides of KR and KL were 1287.82±3.11 mg/L and 336.54±0.00 mg/L respectively. The reducing sugar content from oligosaccharides of KR and KL were 387.33±8.08 mg/L and 144.67±8.08 mg/L. The in vitro analysis of prebiotic activity was performed by Bacterial Growth Assay and Total Plate Counting from Lactobacillus acidophilus and Bifidobacterium longum on each 0, 24, and 48 hours. The Total Plate Counting showed that the maximum bacterial growth on Lactobacillus acidophilus for KR was (8.00±0.28) 108 cfu/mL after 24 hours and (7.35±0.78) 108 cfu/mL for KL after inoculated for 48 hours.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the 6th International Conference of Food, Agriculture, and Natural Resource (IC-FANRES 2021)
Series
Advances in Biological Sciences Research
Publication Date
5 January 2022
ISBN
10.2991/absr.k.220101.025
ISSN
2468-5747
DOI
10.2991/absr.k.220101.025How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

risenwbib
TY  - CONF
AU  - Fitria Susilowati
AU  - Mita Nurul Azkia
PY  - 2022
DA  - 2022/01/05
TI  - Prebiotic Potential of Oligosaccharides: In Vitro Study of Indonesian Local Honey from Apis spp. and Trigona spp. Bees
BT  - Proceedings of the 6th International Conference of Food, Agriculture, and Natural Resource (IC-FANRES 2021)
PB  - Atlantis Press
SP  - 190
EP  - 198
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220101.025
DO  - 10.2991/absr.k.220101.025
ID  - Susilowati2022
ER  -