Optimization of an organic solvents tolerance lipase expressed in E.coli

DOI

10.2991/eesed-16.2017.1How to use a DOI?

Keywords

lipase expression; Pseudomonas aeruginosa; organic solvents tolerance

Abstract

Owing to the safety consideration and improvement of lipase productivity, the genes of lipase and its cognate foldase from Pseudomonas aeruginosa CS-2 was co expressed in E.coli BL2 (DE3) and the optimization of expression condition was investigated in the paper. The recombinant strain entered the stationary phase after 8h. The optimum conditions for the expression of intracellular lipase in E.coli BL21 (DE3) were induction time (6h), the concentration of isopropyl-?-thiogalactopyranoside (1.5mM) and induction temperature (25øC)

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Ren Peng
AU  - Chun-Mei Tong
AU  - Ming-Qing Lin
PY  - 2016/08
DA  - 2016/08
TI  - Optimization of an organic solvents tolerance lipase expressed in E.coli
BT  - 2nd Annual International Conference on Energy, Environmental & Sustainable Ecosystem Development (EESED 2016)
PB  - Atlantis Press
SP  - 1
EP  - 7
SN  - 2352-5401
UR  - https://doi.org/10.2991/eesed-16.2017.1
DO  - 10.2991/eesed-16.2017.1
ID  - Peng2016/08
ER  -