Optimization of an organic solvents tolerance lipase expressed in E.coli
Ren Peng, Chun-Mei Tong, Ming-Qing Lin
Available Online August 2016.
- https://doi.org/10.2991/eesed-16.2017.1How to use a DOI?
- lipase expression; Pseudomonas aeruginosa; organic solvents tolerance
- Owing to the safety consideration and improvement of lipase productivity, the genes of lipase and its cognate foldase from Pseudomonas aeruginosa CS-2 was co expressed in E.coli BL2 (DE3) and the optimization of expression condition was investigated in the paper. The recombinant strain entered the stationary phase after 8h. The optimum conditions for the expression of intracellular lipase in E.coli BL21 (DE3) were induction time (6h), the concentration of isopropyl-?-thiogalactopyranoside (1.5mM) and induction temperature (25øC)
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Ren Peng AU - Chun-Mei Tong AU - Ming-Qing Lin PY - 2016/08 DA - 2016/08 TI - Optimization of an organic solvents tolerance lipase expressed in E.coli BT - 2nd Annual International Conference on Energy, Environmental & Sustainable Ecosystem Development (EESED 2016) PB - Atlantis Press SP - 1 EP - 7 SN - 2352-5401 UR - https://doi.org/10.2991/eesed-16.2017.1 DO - https://doi.org/10.2991/eesed-16.2017.1 ID - Peng2016/08 ER -