2nd Annual International Conference on Energy, Environmental & Sustainable Ecosystem Development (EESED 2016)

Optimization of an organic solvents tolerance lipase expressed in E.coli

Authors
Ren Peng, Chun-Mei Tong, Ming-Qing Lin
Corresponding Author
Ren Peng
Available Online August 2016.
DOI
https://doi.org/10.2991/eesed-16.2017.1How to use a DOI?
Keywords
lipase expression; Pseudomonas aeruginosa; organic solvents tolerance
Abstract
Owing to the safety consideration and improvement of lipase productivity, the genes of lipase and its cognate foldase from Pseudomonas aeruginosa CS-2 was co expressed in E.coli BL2 (DE3) and the optimization of expression condition was investigated in the paper. The recombinant strain entered the stationary phase after 8h. The optimum conditions for the expression of intracellular lipase in E.coli BL21 (DE3) were induction time (6h), the concentration of isopropyl-?-thiogalactopyranoside (1.5mM) and induction temperature (25øC)
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Volume Title
2nd Annual International Conference on Energy, Environmental & Sustainable Ecosystem Development (EESED 2016)
Series
Advances in Engineering Research
Publication Date
August 2016
ISBN
978-94-6252-309-8
ISSN
2352-5401
DOI
https://doi.org/10.2991/eesed-16.2017.1How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Ren Peng
AU  - Chun-Mei Tong
AU  - Ming-Qing Lin
PY  - 2016/08
DA  - 2016/08
TI  - Optimization of an organic solvents tolerance lipase expressed in E.coli
BT  - 2nd Annual International Conference on Energy, Environmental & Sustainable Ecosystem Development (EESED 2016)
PB  - Atlantis Press
SP  - 1
EP  - 7
SN  - 2352-5401
UR  - https://doi.org/10.2991/eesed-16.2017.1
DO  - https://doi.org/10.2991/eesed-16.2017.1
ID  - Peng2016/08
ER  -