Quantitative Detection of raw sheep in Meat Products by a TaqMan Real-Time PCR Assay
Authors
Qiong Xu, Wenjia Gu, Yinan Zhang, Qingping Zhang, Fei Tao, Xuelian Hu, Yan Zhao, Wenfeng Duan, Qinfen Qu
Corresponding Author
Qiong Xu
Available Online July 2015.
- DOI
- 10.2991/cmfe-15.2015.10How to use a DOI?
- Keywords
- real-time PCR; quantitative detection; raw; Sheep; adulteration
- Abstract
In the present work, we propose a normalised real-time quantitative PCR assay to determine the sheep content in raw meat. For the development of the method, the mixtures meats contain known amounts of sheep meat in pork from 20% to 100% can be successfully in-house validated by a normalised calibration model with high linear correlation (R2=0.994) and PCR efficiency (E=77%). The method also showed that the limit of detection of sheep in sheep-pork mixtures was 0.8% (W/W). Thus, this methodology could serve as a fast and sensitive method for quantitative detection of sheep for meat species verification, avoiding fraudulent practices.
- Copyright
- © 2015, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Qiong Xu AU - Wenjia Gu AU - Yinan Zhang AU - Qingping Zhang AU - Fei Tao AU - Xuelian Hu AU - Yan Zhao AU - Wenfeng Duan AU - Qinfen Qu PY - 2015/07 DA - 2015/07 TI - Quantitative Detection of raw sheep in Meat Products by a TaqMan Real-Time PCR Assay BT - Proceedings of the International Conference on Chemical, Material and Food Engineering PB - Atlantis Press SP - 39 EP - 42 SN - 2352-5401 UR - https://doi.org/10.2991/cmfe-15.2015.10 DO - 10.2991/cmfe-15.2015.10 ID - Xu2015/07 ER -