Features of Immunological Indicators in Workers Under the Influence of Factors of the Production Environment
- DOI
- 10.2991/ahsr.k.220103.006How to use a DOI?
- Keywords
- immunological indicators; environment; immune regulation; monitoring of workers
- Abstract
An assessment of the immune regulation, its cellular and humoral components was carried out for diagnosing early health disorders in potassium production workers affected by excessive contamination of biological media with formaldehyde. The purpose of the article is to study the influence of chemical factors of the working environment on the parameters of immune regulation in workers of the mining industry. The observation group included 157 people aged 43.2 ± 1.3 years working in hazardous working conditions. The control group included 78 patients aged 44.5 ± 1.2 years working as managers of the company. Phagocytosis was assessed using formalized sheep erythrocytes. Serum immunoglobulins A, M, G were determined by the method of radial immunodiffusion according to Mancini. Specific IgE to formaldehyde was determined by the allergosorbent test with an enzyme label. Total IgE, interleukin-1β, tumor necrosis factor were determined using enzyme-linked immunosorbent assay. CD lymphocytes were phenotyped by flow cytometry. Populations and subpopulations of lymphocytes (CD16 +, CD19 +, CD25 +, CD95 +) were determined by the method of membrane immunofluorescence. The significance of differences between the groups was considered significant at p <0.05. According to the results of chemical analytical studies, an excess of the permissible level of formaldehyde in the blood was observed both in the observation group and in the comparison group. There was a significant decrease in the phagocytic activity according to the criterion of absolute phagocytosis (p≤0.05). A significantly increased content of serum immunoglobulins G, A, M was established. At the same time, an increased level of general sensitization and an increased level of specific sensitization to formaldehyde were revealed in 21.8 % of workers (p <0.05). An increased expression of the killer lymphocyte receptors CD16 + CD56 + and CD19 + was revealed (p <0.05). Activation of cell death receptors CD95 + and CD25 + was also observed (p <0.05). An excess of the level of IL-1β and a decrease in the level of TNF relative to the control group (p <0.05) were observed. The content of the mediator of catecholamine regulation of serotonin was lower in the observation group, and the hormone of the adrenal cortex cortisol was higher. According to the results of an immunological study of mining workers, deviations in the system of humoral and cellular immunity in the form of a decrease in the phagocytic activity, an increase in the content of serum immunoglobulins, the level of general and specific sensitization, increased expression of killer lymphocytes CD19 + and CD16 + CD56 + and cell death receptors CD25 + and CD95 + were revealed. An increase in the content of IL-1β and inhibition of TNF were observed. A decrease in the level of expression of serotonin and an increase in cortisol were observed as well. It is necessary to use high-tech research methods in the sanitary and epidemiological surveillance and immunological monitoring of workers exposed to man-made pollutants.
- Copyright
- © 2022 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article under the CC BY-NC license.
Cite this article
TY - CONF AU - Yu.A. Chelakova AU - O.V Dolgikh AU - V.B. Alekseev PY - 2022 DA - 2022/01/17 TI - Features of Immunological Indicators in Workers Under the Influence of Factors of the Production Environment BT - Proceedings of the Conference on Health and Wellbeing in Modern Society (CHW 2021) PB - Atlantis Press SP - 26 EP - 30 SN - 2468-5739 UR - https://doi.org/10.2991/ahsr.k.220103.006 DO - 10.2991/ahsr.k.220103.006 ID - Chelakova2022 ER -