Differences between Aureobasidium Pullulans CGMCC No.3337 and Aureobasidium Pullulans TKPM10017 on Synthesis of Poly (β-L-malic-acid) Metabolism

DOI

10.2991/bst-16.2016.10How to use a DOI?

Keywords

Poly (β-L-malic-acid) (PMLA), Aureobasidium pullulans, Protein spectrum experiment, Intracellular enzyme activity.

Abstract

Poly (β-L-malic-acid) (PMLA) is a sort of polyester and absolute biodegradable material. PMLA is a new type of nontoxic macromolecule material and with favorable biodegradation and biocompatibility. PMLA has extensive applications in the areas of pharmacy and medical industry. Application foreground of PMLA is very extensive. Poly malic acid has widely attracted the attention of people, but the studies of poly malic acid - Aureobasidium pullulans (A.pullulans) and the metabolic mechanism of A.pullulans is rarely reported, the researchers abroad studied A.pullulans is earlier than domestic researchers. The Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science and Technology preserve two strains which have different production--Aureobasidium pullulans CGMCC No.3337 and Aureobasidium pullulans TKPM10017. A.pullulans CGMCC No.3337 was selected from A.pullulans TKPM10017 by UV and He-Ne laser. There were significant differences in production of PMLA. In order to study the significant differences, we carried out the protein spectrum experiment the extraction of genome of A.pullulans TKPM10017 and A.pullulans CGMCC No.3337 and the Polymerase Chain Reaction of ATP synthase alpha subunit and ATP synthase beta subunit and gene sequence analysis. From the metabolic pathway perspective analyze on the causes of such differences.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Hai-Song Yin
AU  - Yan-Hui Bian
AU  - Wei-Hua Tang
AU  - Xu-Jia Fan
AU  - Zheng Li
AU  - Chang-Sheng Qiao
PY  - 2016/01
DA  - 2016/01
TI  - Differences between Aureobasidium Pullulans CGMCC No.3337 and Aureobasidium Pullulans TKPM10017 on Synthesis of Poly (β-L-malic-acid) Metabolism
BT  - Proceedings of the 2016 International Conference on Biological Sciences and Technology
PB  - Atlantis Press
SP  - 53
EP  - 61
SN  - 2468-5747
UR  - https://doi.org/10.2991/bst-16.2016.10
DO  - 10.2991/bst-16.2016.10
ID  - Yin2016/01
ER  -