Cloning and Expression of cry9Ea10 Gene from Bacillus Thuringiensis Strain GZ2 Isolated from Infected Hyphantria Cunea Larvae

DOI

10.2991/bbe-16.2016.38How to use a DOI?

Keywords

Bacillus thuringiensis, Cry gene,Infected Hyphantria cunea, Biological control.

Abstract

A novelBacillus thuringiensis(Bt) strain GZ2, was isolated from infected Hyphantria cunealarvae. The bioassay results showed that GZ2 had insecticidal activity to H.cunea larvae. The genotypes ofGZ2 were identified by PCR-RFLP method. GZ2 was identified to harbor cry9Ea10 gene and the full length of cry9Ea10 was cloned and sequenced. The engineering strain of Bt carrying cry9Ea10gene wasconstructed successfully. SDS-PAGE analysis confirmed that the expression of cry9Ea10 as ~130kDa protein. And Cry9Ea10 protein produced by Bt engineeringstrainexhibited a high toxicity to H.cunealarvae.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Kunli Zhao
AU  - Wei Guo
AU  - Dan Zhao
AU  - Yakun Zhang
AU  - Xiaoping Yan
AU  - Yujie Gao
PY  - 2016/07
DA  - 2016/07
TI  - Cloning and Expression of cry9Ea10 Gene from Bacillus Thuringiensis Strain GZ2 Isolated from Infected Hyphantria Cunea Larvae
BT  - Proceedings of the 2016 International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SP  - 226
EP  - 231
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.38
DO  - 10.2991/bbe-16.2016.38
ID  - Zhao2016/07
ER  -