The Effect of Light and Medium on Secondary Metabolite Production in Callus Culture of Kaemferia galanga Linn

DOI

10.2991/amca-18.2018.88How to use a DOI?

Keywords

Sucrose, plant regulators, light, callus culture, Kaempferia galanga

Abstract

This research investigated the effects of different media and environmental treatment (light and dark) on biomass and secondary metabolite production in callus culture of Kaemferia galanga. Environmental light had no significant effect on biomass callus. Media with sucrose 30 g/L under light condition gave the highest biomass callus. Kaemferia galanga L callus contained alkaloids, flavonoids, saponins, steroids and ethyl p-methoxycinnamate (EPMC). Mean of EPMC content in bright environmental condition with sucrose concentration 20 g / L medium gave average concentration value of ethyl p-methoxycinnamate 7,49 g / L and with addition of sucrose 30 - 40 g / L an increase of 2.31 to 2,39 times While the environmental treatment without light (dark) with a sucrose concentration of 20 g / L medium of 3.21 g / L, with sucrose concentration added up to 30-40 g / L there was an increase of ethyl p-methoxycinnamate levels ranging from 1.18 to 2.01 times

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Anis Shofiyani
PY  - 2018/07
DA  - 2018/07
TI  - The Effect of Light and Medium on Secondary Metabolite Production in Callus Culture of Kaemferia galanga Linn
BT  - Proceedings of the 5th International Conference on Community Development (AMCA 2018)
PB  - Atlantis Press
SP  - 322
EP  - 325
SN  - 2352-5398
UR  - https://doi.org/10.2991/amca-18.2018.88
DO  - 10.2991/amca-18.2018.88
ID  - Shofiyani2018/07
ER  -