Features of Seeds Microbiome for Spring Wheat Varieties from Different Regions of Eurasia

DOI

10.2991/agrosmart-18.2018.143How to use a DOI?

Keywords

metagenomic analysis, bacterial endophytes, biological agents, biofungicides, Fusarium oxysporum, spring wheat, seeds

Abstract

The aim of the study was to investigate the peculiarities of microbiome and seed bacterial endophytes isolated from different varieties of spring wheat grown in different areas of Eurasia (European part of Russia (Volga region), countries of Central Asia). The main method of the microbiome studies was PCR. Isolation of bacterial endophytes from the seeds allowed estimating their count and evaluating their activity towards Fusarium oxysporum. Quantitative analysis of the microbiome was performed on seeds from different spring wheat varieties (genotypes) obtained from the Russian Federation, the Republic of Kazakhstan and the Republic of Tadzhikistan. The differences in the bacterial endophytic microflora of the seeds and their activity towards Fusarium oxysporum were established. The seeds grown in Central Asia have higher count of both total microbiome and bacterial endophytes active against phytopathogens than the seeds grown in Volga region of the Russian Federation. Differences in the species composition of seed endophytic bacteria were established. Active strains of seed endophytic bacteria obtained from Tajikistan belonged to the species Bacillus jeuvensis. Active strains of bacterial endophytes were isolated from spring wheat seeds grown in Tadzhikistan for the development of highly potential biofungicides. The study results confirmed that seed microbiome depends on variety and the area of cultivation. The materials of the study can be useful for the evaluation of the seeds in the breeding of spring wheat and in the search for new biological agents for biofungicides development.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - R.I. Safin
AU  - L.Z. Karimova
AU  - R.M. Nizamov
AU  - A.R. Valiev
AU  - S.Z. Validov
AU  - D.I. Faizrakhmanov
PY  - 2018/12
DA  - 2018/12
TI  - Features of Seeds Microbiome for Spring Wheat Varieties from Different Regions of Eurasia
BT  - International scientific and practical conference "AgroSMART - Smart solutions for agriculture" (AgroSMART 2018)
PB  - Atlantis Press
SP  - 766
EP  - 770
SN  - 2352-5401
UR  - https://doi.org/10.2991/agrosmart-18.2018.143
DO  - 10.2991/agrosmart-18.2018.143
ID  - Safin2018/12
ER  -