Identification of Phytotoxic and Phytopathogenic Fungi on Grains and Wheat Seedlings

DOI

10.2991/agrosmart-18.2018.142How to use a DOI?

Keywords

fungal plant disease, mycotoxins, phytopathogen detection, qPCR, Alternaria sp.

Abstract

Fungal phytopathogens are responsible for significant losses in agriculture worldwide. Diagnosis of seed infestation by fungal strains causing plant diseases or producing toxins is important for sustainable agriculture. Since plants are always colonized by fungal strains there is a demand in approaches for reliable discrimination between harmless and hazardous fungi both in grains. We tested phytotoxic properties, mycotoxin production and colonization of wheat seedlings among 11 fungal isolated obtained from wheat grains of different varieties. The isolates were identified as Alternaria tenuissima, Alternaria infectoria, Bipolaris sorokiniana Alternaria arborescens, Alternaria alternata and Alternaria rosae. Strains A. tenuissima AB-F 72, A. arborescens AB-F 73, A. alternata AB-F 75, A. rosae AB-F 77 were producing phytotoxins inhbiting growth of wheat seedling. Four strains: B. sorokiniana AB-F 61, A. arborescens AB-F 73, A. alternata AB-F 74, A. rosae AB-F 77 were producing mycotoxins. Five strains A. rosae AB-F 77, A. tenuissima AB-F 76, A. infectoria AB-F 78 and A. infectoria AB-F 84 showed heavy colonization of wheat plantlets suggesting that these strains can be pathogenic to wheat. The results show that toxin production is not an obligatory characteristic for phytopathogenic strain, therefore using of proliferation of fungal strain in seedling tissue can be added as an additional test to seed quality evaluation.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - A.V. Pankova
AU  - L.R. Valiullin
AU  - D.V. Afordoanyi
AU  - R.I. Safin
AU  - A.R. Valiev
AU  - R.M. Nizamov
AU  - S.Z. Validov
PY  - 2018/12
DA  - 2018/12
TI  - Identification of Phytotoxic and Phytopathogenic Fungi on Grains and Wheat Seedlings
BT  - International scientific and practical conference "AgroSMART - Smart solutions for agriculture" (AgroSMART 2018)
PB  - Atlantis Press
SP  - 759
EP  - 765
SN  - 2352-5401
UR  - https://doi.org/10.2991/agrosmart-18.2018.142
DO  - 10.2991/agrosmart-18.2018.142
ID  - Pankova2018/12
ER  -