Three-Dimensional Model of a Novel Photosynthetic Ferredoxin Protein from Gardenia jasminoides

DOI

10.2991/aeecs-18.2018.7How to use a DOI?

Keywords

Chloroplast ferredoxins; photosyntheie; Gardenia jasminoides; cDNA library; Three-Dimensional Model.

Abstract

Chloroplast ferredoxins (Fd) are small proteins containing an iron-sulfur cluster in photosynthetic organisms. Fd functions as transferring electrons from photosystem I (PSI) to NADP+ via ferredoxin: NADPH+ reductase (FNR). Fd is also the low-potential electron donor to many metabolic and regulatory pathways of the chloroplast. It plays a dual role in primary and secondary metabolism. In this work, we aimed to isolate a gene of Fd from Gardenia jasminoides. A G. jasminoides fruit cDNA library was constructed, and the GjFd cDNA was isolated from the cDNA library by sequencing method. The GjFd cDNA contains a predicted 444 bp open reading frame that encodes 147 amino acids. A bioinformatics analysis indicated that GjFd has conserved four iron-binding sites and FNR binding residues. A three-dimensional dimmer model of GjFd was built, the structure is similar to Fds from maize and spinach. The results suggest that GjFd is a typical photosynthetic ferredoxin protein mainly responsible for linear photosynthetic electron transort pathway.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Lan Gao
PY  - 2018/03
DA  - 2018/03
TI  - Three-Dimensional Model of a Novel Photosynthetic Ferredoxin Protein from Gardenia jasminoides
BT  - Proceedings of the 2018 2nd International Conference on Advances in Energy, Environment and Chemical Science (AEECS 2018)
PB  - Atlantis Press
SP  - 28
EP  - 32
SN  - 2352-5401
UR  - https://doi.org/10.2991/aeecs-18.2018.7
DO  - 10.2991/aeecs-18.2018.7
ID  - Gao2018/03
ER  -