Proceedings of the 2018 2nd International Conference on Advances in Energy, Environment and Chemical Science (AEECS 2018)

Structure of a Novel Rubisco Activase in Gardenia jasminoides

Authors
Lan Gao
Corresponding Author
Lan Gao
Available Online March 2018.
DOI
10.2991/aeecs-18.2018.8How to use a DOI?
Keywords
Rubisco; Rubisco activase; Gardenia jasminoides; cDNA library; 3D-structure.
Abstract

The key photosynthetic CO2-fixing enzyme ribulose-1,5-bisphosphate carboxylase/ oxygenase (Rubisco) is regulated by a protein known as Rubisco activase (RCA). RCA is a soluble chloroplast ATPase encoded by nuclear genome that catalyzes the activation of Rubisco in vivo by the ATP-dependent removal of various inhibitory sugar phosphates. In this work, we constructed a G.jasminoides fruit cDNA library, and the GjRCA cDNA was isolated from the library by sequencing method. The GjRCA cDNA contains a predicted 1428 bp open reading frame that encodes 475 amino acids. A bioinformatics analysis was conducted with previously characterized RCAs from other plant species, the results suggested that the GjRCA amino acid sequence has highly conserved nucleotide-binding region, critical ATPase amino acid residue and Rubisco-interaction region with other RCAs. A three-dimensional monomer model of GjRCA was built, GjRCA has extended carboxyl terminus sequence with two conserved cysteine residues, and has 46 kD molecular weight, suggest it's a L-isoform of RCA, it's similar to structure of AAA+ family protein and other RCAs. Conclusion: The results indicated that GjRCA is a L-isofrom RCA of G. jasminoides.

Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2018 2nd International Conference on Advances in Energy, Environment and Chemical Science (AEECS 2018)
Series
Advances in Engineering Research
Publication Date
March 2018
ISBN
978-94-6252-479-8
ISSN
2352-5401
DOI
10.2991/aeecs-18.2018.8How to use a DOI?
Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Lan Gao
PY  - 2018/03
DA  - 2018/03
TI  - Structure of a Novel Rubisco Activase in Gardenia jasminoides
BT  - Proceedings of the 2018 2nd International Conference on Advances in Energy, Environment and Chemical Science (AEECS 2018)
PB  - Atlantis Press
SP  - 33
EP  - 36
SN  - 2352-5401
UR  - https://doi.org/10.2991/aeecs-18.2018.8
DO  - 10.2991/aeecs-18.2018.8
ID  - Gao2018/03
ER  -