eFood

Volume 1, Issue 6, December 2020, Pages 406 - 409

Eukaryotic Expression and Purification Technology of Glucosidase in Pichia Pastoris

Authors
Weijie Zhou, Dongsheng Yao, Weibin Bai*
Department of Food Science and Engineering, Institute of Food Safety and Nutrition, Guangdong Engineering Technology Center of Food Safety Molecular Rapid Detection, Jinan University, Guangzhou, China
*Corresponding author. Email: baiweibin@163.com
Corresponding Author
Weibin Bai
Received 27 November 2020, Accepted 29 December 2020, Available Online 8 January 2021.
DOI
https://doi.org/10.2991/efood.k.201227.001How to use a DOI?
Keywords
Pichia pastoris, glucosidase, eukaryotic expression, protein purification, p-NPG
Abstract

Glucosidase is one of the most important hydrolases in the carbohydrate metabolism pathway in human body. It is noteworthy that glucosidase was raising high attention in numerous researches, attributed to its functional values and biological activities. The expression, secretion, extraction, purification, and quantitative analysis of glucosidase occupies an important position in the research of glucosidase. The Pichia pastoris expression system has been developed for 50 years. With its unique advantages, the expression system has successfully expressed hundreds of heterologous proteins. This article briefly describes the process of high-efficiency expression, extraction and purification of glucosidase in the P. pastoris expression system, focusing on the expression, separation and purification of glucosidase in P. pastoris, as well as the quantitative and activity analysis methods of glucosidase. Affinity chromatography is used for the separation and purification of crude enzyme solution, and UV–visible spectroscopy can be used for enzyme quantification and activity analysis.

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Copyright
© 2021 The Authors. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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Journal
eFood
Volume-Issue
1 - 6
Pages
406 - 409
Publication Date
2021/01
ISSN (Online)
2666-3066
DOI
https://doi.org/10.2991/efood.k.201227.001How to use a DOI?
Copyright
© 2021 The Authors. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - JOUR
AU  - Weijie Zhou
AU  - Dongsheng Yao
AU  - Weibin Bai
PY  - 2021
DA  - 2021/01
TI  - Eukaryotic Expression and Purification Technology of Glucosidase in Pichia Pastoris
JO  - eFood
SP  - 406
EP  - 409
VL  - 1
IS  - 6
SN  - 2666-3066
UR  - https://doi.org/10.2991/efood.k.201227.001
DO  - https://doi.org/10.2991/efood.k.201227.001
ID  - Zhou2021
ER  -