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Comprehensive Polyphenolic Profile of Plantago depressa using High-Speed Countercurrent Chromatography Off-line with High-Performance Liquid Chromatography–Diode Array Detector–Quadrupole Time-of-flight Tandem Mass Spectrometry

Authors
Jinju Xu1, Chaoying Tong1, *, Qiachi Fu1, Keke Guo1, Shuyun Shi1, 2, *, Yecheng Xiao2
1Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China
2Research and Development Department, Lianyuan Kanglu Biotech Co., Ltd., Lianyuan 417100, PR China
*Corresponding authors. Email: tcycsu@csu.edu.cn; shishuyun@126.com
Corresponding Authors
Chaoying Tong, Shuyun Shi
Received 20 April 2019, Accepted 29 October 2019, Available Online 14 November 2019.
DOI
10.2991/efood.k.191101.001How to use a DOI?
Keywords
Plantago depressa; polyphenol; high-speed countercurrent chromatography; high-performance liquid chromatography; tandem mass spectrometry
Abstract

The whole plant of Plantago depressa is commonly used as traditional Chinese medicine and functional food ingredient. Total phenolic content and total flavonoid content evaluation indicated that its ethyl acetate and n-butanol fractions were rich in polyphenols. However, to date, the polyphenolic profile is limited because of the complex matrix. Here, polyphenols in ethyl acetate and n-butanol fractions of P. depressa were first separated and enriched by High-Speed Countercurrent Chromatography (HSCCC). Then, their structures were characterized or tentatively identified by High-Performance Liquid Chromatography (HPLC)–Diode Array Detector (DAD)–Quadrupole Time-of-Flight Tandem Mass Spectrometry (QTOF-MS/MS) (HSCCC × HPLC–DAD–QTOF-MS/MS). Because of the orthogonality of HSCCC and HPLC, 37 polyphenols were identified. Notably, 29 polyphenols were discovered from P. depressa for the first time. Finally, contents of two potential new compounds, 2-ethoxy plantamajoside isomers (17 and 29), were determined as 0.27 ± 0.03 and 0.04 ± 0.01 mg/g, respectively. Results indicated that P. depressa could be used as a promising source of polyphenols for health promotion, and the developed method (HSCCC × HPLC) was efficient to explore polyphenolic profile for complex natural products.

Copyright
© 2019 International Association of Dietetic Nutrition and Safety. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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<Previous Article In Issue
Journal
eFood
Volume-Issue
1 - 1
Pages
94 - 105
Publication Date
2019/11/14
ISSN (Online)
2666-3066
DOI
10.2991/efood.k.191101.001How to use a DOI?
Copyright
© 2019 International Association of Dietetic Nutrition and Safety. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - JOUR
AU  - Jinju Xu
AU  - Chaoying Tong
AU  - Qiachi Fu
AU  - Keke Guo
AU  - Shuyun Shi
AU  - Yecheng Xiao
PY  - 2019
DA  - 2019/11/14
TI  - Comprehensive Polyphenolic Profile of Plantago depressa using High-Speed Countercurrent Chromatography Off-line with High-Performance Liquid Chromatography–Diode Array Detector–Quadrupole Time-of-flight Tandem Mass Spectrometry
JO  - eFood
SP  - 94
EP  - 105
VL  - 1
IS  - 1
SN  - 2666-3066
UR  - https://doi.org/10.2991/efood.k.191101.001
DO  - 10.2991/efood.k.191101.001
ID  - Xu2019
ER  -