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Volume 1, Issue 2, April 2020, Pages 147 - 155

Xanthine Oxidase Inhibitors Screening, Antioxidation, and DNA Protection Properties of Geranium wilfordii Maxim

Authors
Yi Liu†, Shiyong Mei†, Aiping Xiao, Liangliang Liu*
Laboratory of Quality & Safety Risk Assessment for Vegetable fiber Products, Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China
†

Yi Liu and Shiyong Mei contributed equally to this work.

*Corresponding author. Email: liuliangliang@caas.cn
Corresponding Author
Liangliang Liu
Received 22 June 2019, Accepted 9 July 2019, Available Online 23 August 2019.
DOI
10.2991/efood.k.190802.004How to use a DOI?
Keywords
Antioxidation; DNA damage; DPPH spiking; inhibitor; ultrafiltration; xanthine oxidase
Abstract

Geranium wilfordii Maxim is a commonly used traditional medicine and as decoction pieces in drinks for treating acute and chronic rheumatalgia. Earlier research showed it could inhibit xanthine oxidase and had a strong antioxidative activity. In this study, four compounds namely, corilagin, geraniin, ellagic acid, and myricetrin were screened and identified as xanthine oxidase inhibitors and antioxidants using ultrafiltration combined liquid chromatography–mass spectrometry (LC–MS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) spiking combined LC–MS methods. Ellagic acid showed the greatest inhibition with IC50 of 0.018 ± 0.001 mM better than that of allopurinol. The kinetic parameters exhibited the mode of xanthine oxidase inhibitions by corilagin, geraniin, and myricetrin were the competitive types, whereas the ellagic acid was found as an uncompetitive inhibitor. In vitro DNA nicking assay showed these four compounds protected DNA from reactive oxygen species. Correlation analysis and partial least squares analysis showed there was a moderate correlation between ellagic acid and DPPH˙ scavenging activity. Our findings revealed four compounds in G. wilfordii Maxim contributed satisfied radicals scavenging, xanthine oxidase inhibition, and DNA damage protective activities. More comprehensive research on pharmaceutical applications and phytochemical profile of G. wilfordii Maxim could be further studied.

Copyright
© 2019 International Association of Dietetic Nutrition and Safety. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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<Previous Article In Issue
Next Article In Issue>
Journal
eFood
Volume-Issue
1 - 2
Pages
147 - 155
Publication Date
2019/08/23
ISSN (Online)
2666-3066
DOI
10.2991/efood.k.190802.004How to use a DOI?
Copyright
© 2019 International Association of Dietetic Nutrition and Safety. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - JOUR
AU  - Yi Liu
AU  - Shiyong Mei
AU  - Aiping Xiao
AU  - Liangliang Liu
PY  - 2019
DA  - 2019/08/23
TI  - Xanthine Oxidase Inhibitors Screening, Antioxidation, and DNA Protection Properties of Geranium wilfordii Maxim
JO  - eFood
SP  - 147
EP  - 155
VL  - 1
IS  - 2
SN  - 2666-3066
UR  - https://doi.org/10.2991/efood.k.190802.004
DO  - 10.2991/efood.k.190802.004
ID  - Liu2019
ER  -