Monocyte Reconstitution and Gut Microbiota Composition after Hematopoietic Stem Cell Transplantation

Authors

Sejal Morjaria^{1, 7, *,} , Allen W. Zhang², Sohn Kim³, Jonathan U. Peled^{6, 7}, Simone Becattini³, Eric R. Littmann³, Eric. G. Pamer^{1, 3, 4, 7}, Michael C. Abt^{5, *, †,} , Miguel-Angel Perales^{6, 7, †}

¹Infectious Disease, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA

²Department of Epidemiology and Biostatistics, Memorial Sloan Kettering Cancer Center, New York, NY, USA

³Immunology Program and Infectious Disease Service, Memorial Sloan-Kettering Cancer Center, New York, NY, USA

⁴Lucielle Castori Center for Microbes, Inflammation and Cancer, Sloan Kettering Institute, New York, NY, USA

⁵Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA

⁶Adult Bone Marrow Transplantation Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA

⁷Department of Medicine, Weill Cornell Medical College, New York, NY, USA

^†

MP and MA are senior co-authors.

^*Corresponding authors. Email: morjaris@mskcc.org; michael.abt@pennmedicine.upenn.edu

DOI

10.2991/chi.k.201108.002How to use a DOI?

Keywords

Immune reconstitution; monocytes; microbiome; bone marrow transplantation

Abstract

Background: Monocytes are an essential cellular component of the innate immune system that support the host’s effectiveness to combat a range of infectious pathogens. Hemopoietic cell transplantation (HCT) results in transient monocyte depletion, but the factors that regulate recovery of monocyte populations are not fully understood. In this study, we investigated whether the composition of the gastrointestinal microbiota is associated with the recovery of monocyte homeostasis after HCT.

Methods: We performed a single-center, prospective, pilot study of 18 recipients of either autologous or allogeneic HCT. Serial blood and stool samples were collected from each patient during their HCT hospitalization. Analysis of the gut microbiota was done using 16S rRNA gene sequencing, and flow cytometric analysis was used to characterize the phenotypic composition of monocyte populations.

Results: Dynamic fluctuations in monocyte reconstitution occurred after HCT, and large differences were observed in monocyte frequency among patients over time. Recovery of absolute monocyte counts and subsets showed significant variability across the heterogeneous transplant types and conditioning intensities; no relationship to the microbiota composition was observed in this small cohort.

Conclusion: In this pilot study, a relationship between the microbiota composition and monocyte homeostasis could not be firmly established. However, we identify multivariate associations between clinical factors and monocyte reconstitution post-HCT. Our findings encourage further longitudinal surveillance of the intestinal microbiome and its link to immune reconstitution.

Copyright

© 2020 International Academy for Clinical Hematology. Publishing services by Atlantis Press International B.V.

Open Access

This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)
View full text (HTML)

TY  - JOUR
AU  - Sejal Morjaria
AU  - Allen W. Zhang
AU  - Sohn Kim
AU  - Jonathan U. Peled
AU  - Simone Becattini
AU  - Eric R. Littmann
AU  - Eric. G. Pamer
AU  - Michael C. Abt
AU  - Miguel-Angel Perales
PY  - 2020
DA  - 2020/11/23
TI  - Monocyte Reconstitution and Gut Microbiota Composition after Hematopoietic Stem Cell Transplantation
JO  - Clinical Hematology International
SP  - 156
EP  - 164
VL  - 2
IS  - 4
SN  - 2590-0048
UR  - https://doi.org/10.2991/chi.k.201108.002
DO  - 10.2991/chi.k.201108.002
ID  - Morjaria2020
ER  -