Clinical Hematology International

Volume 2, Issue 4, December 2020, Pages 156 - 164

Monocyte Reconstitution and Gut Microbiota Composition after Hematopoietic Stem Cell Transplantation

Authors
Sejal Morjaria1, 7, *, ORCID, Allen W. Zhang2, Sohn Kim3, Jonathan U. Peled6, 7, Simone Becattini3, Eric R. Littmann3, Eric. G. Pamer1, 3, 4, 7, Michael C. Abt5, *, , ORCID, Miguel-Angel Perales6, 7,
1Infectious Disease, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA
2Department of Epidemiology and Biostatistics, Memorial Sloan Kettering Cancer Center, New York, NY, USA
3Immunology Program and Infectious Disease Service, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
4Lucielle Castori Center for Microbes, Inflammation and Cancer, Sloan Kettering Institute, New York, NY, USA
5Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
6Adult Bone Marrow Transplantation Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA
7Department of Medicine, Weill Cornell Medical College, New York, NY, USA

MP and MA are senior co-authors.

Corresponding Author
Sejal Morjaria
Received 7 September 2020, Accepted 21 October 2020, Available Online 23 November 2020.
DOI
https://doi.org/10.2991/chi.k.201108.002How to use a DOI?
Keywords
Immune reconstitution, monocytes, microbiome, bone marrow transplantation
Abstract

Background: Monocytes are an essential cellular component of the innate immune system that support the host’s effectiveness to combat a range of infectious pathogens. Hemopoietic cell transplantation (HCT) results in transient monocyte depletion, but the factors that regulate recovery of monocyte populations are not fully understood. In this study, we investigated whether the composition of the gastrointestinal microbiota is associated with the recovery of monocyte homeostasis after HCT.

Methods: We performed a single-center, prospective, pilot study of 18 recipients of either autologous or allogeneic HCT. Serial blood and stool samples were collected from each patient during their HCT hospitalization. Analysis of the gut microbiota was done using 16S rRNA gene sequencing, and flow cytometric analysis was used to characterize the phenotypic composition of monocyte populations.

Results: Dynamic fluctuations in monocyte reconstitution occurred after HCT, and large differences were observed in monocyte frequency among patients over time. Recovery of absolute monocyte counts and subsets showed significant variability across the heterogeneous transplant types and conditioning intensities; no relationship to the microbiota composition was observed in this small cohort.

Conclusion: In this pilot study, a relationship between the microbiota composition and monocyte homeostasis could not be firmly established. However, we identify multivariate associations between clinical factors and monocyte reconstitution post-HCT. Our findings encourage further longitudinal surveillance of the intestinal microbiome and its link to immune reconstitution.

Copyright
© 2020 International Academy for Clinical Hematology. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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Journal
Clinical Hematology International
Volume-Issue
2 - 4
Pages
156 - 164
Publication Date
2020/11
ISSN (Online)
2590-0048
DOI
https://doi.org/10.2991/chi.k.201108.002How to use a DOI?
Copyright
© 2020 International Academy for Clinical Hematology. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - JOUR
AU  - Sejal Morjaria
AU  - Allen W. Zhang
AU  - Sohn Kim
AU  - Jonathan U. Peled
AU  - Simone Becattini
AU  - Eric R. Littmann
AU  - Eric. G. Pamer
AU  - Michael C. Abt
AU  - Miguel-Angel Perales
PY  - 2020
DA  - 2020/11
TI  - Monocyte Reconstitution and Gut Microbiota Composition after Hematopoietic Stem Cell Transplantation
JO  - Clinical Hematology International
SP  - 156
EP  - 164
VL  - 2
IS  - 4
SN  - 2590-0048
UR  - https://doi.org/10.2991/chi.k.201108.002
DO  - https://doi.org/10.2991/chi.k.201108.002
ID  - Morjaria2020
ER  -