Artery Research

Volume 27, Issue 1, March 2021, Pages 38 - 46

Primary African American Endothelial Cells Exhibit Endothelial Dysfunction with an Exacerbated Inflammatory Profile and Blunted MMP-2 Activity

Authors
Marc D. Cook1, 7, *, ORCID, Chenyi Ling2, ORCID, Heather Grimm3, Adelola Adeyemo6, ORCID, Maitha Aldokhayyil4, ORCID, Kevin Heffernan6, Bo Fernhall7, Michael Brown4, 7
1Department of Kinesiology, North Carolina Agricultural and Technical State University, Greensboro, NC, USA
2Department of Biology, University of Alaska, Fairbanks, AK, USA
3Department of Exercise Science, Kings College, Wilkes-Barre, PA, USA
4School of Kinesiology, Auburn University, Auburn, AL, USA
5School of Medicine-Geriatrics, University of Utah, Salt Lake City, UT, USA
6Department of Exercise Science, Syracuse University, Syracuse, NY, USA
7Department of Kinesiology and Nutrition, University of Illinois at Chicago, Chicago, IL, USA
*Corresponding author. Email: mdcook@ncat.edu
Corresponding Author
Marc D. Cook
Received 26 February 2020, Accepted 3 November 2020, Available Online 18 November 2020.
DOI
https://doi.org/10.2991/artres.k.201102.005How to use a DOI?
Keywords
Endothelial dysfunction, hypertension, inflammation, matrix metalloproteinase-2, racial difference
Abstract

Endothelial dysfunction is associated with the racial health disparity in vascular dysfunction in African Americans (AAs). Matrix Metalloproteinase (MMP)-2 is constitutively expressed in endothelial cells (EC) and is a biomarker that has been associated with hypertension, as its properties are involved in pathologic oxidative stress and pro-inflammation that may affect vascular homeostasis. Herein, we report significant inverse relationships between MMP-2, stroke volume, carotid and aortic systolic pressures in a small cohort of young AA men. In the current study, we postulated that basal activation in AA Endothelial Cells (EC) may include different responses in MMP-2 activity, compared to Caucasian (CA). We evaluated gene and protein expression and activity of MMP-2, and related peptides, in multiple different primary Human Umbilical Vein Endothelial Cells (HUVEC) isolated from four different AA and CA donors. Compared to CA, AA HUVEC exhibited greater basal MMP-2, MMP-14, Tissue inhibitor of metalloproteinase-2, Vascular cell adhesion molecule-1, Intracellular adhesion molecule-1, and Interleukin (IL)-1β gene expression and greater endothelin-1 secretion (p < 0.05). Interestingly, basal MMP-2 protein expression was greater while relative secreted MMP-2 activity was lower (p = 0.041). Inflammatory stimuli (tumor necrosis factor-alpha) exacerbated relative MMP-2 activity in AA HUVEC (p = 0.007). These in vitro data offer insights into a potential mechanism involving primary endothelial cell inflammatory mediated MMP-2 activities that may contribute to poorer vascular outcomes. Further studies are necessary to investigate endothelial intracellular transcriptional, translational, and activity regulation of MMP-2.

Copyright
© 2020 Association for Research into Arterial Structure and Physiology. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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Journal
Artery Research
Volume-Issue
27 - 1
Pages
38 - 46
Publication Date
2020/11
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
https://doi.org/10.2991/artres.k.201102.005How to use a DOI?
Copyright
© 2020 Association for Research into Arterial Structure and Physiology. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - JOUR
AU  - Marc D. Cook
AU  - Chenyi Ling
AU  - Heather Grimm
AU  - Adelola Adeyemo
AU  - Maitha Aldokhayyil
AU  - Kevin Heffernan
AU  - Bo Fernhall
AU  - Michael Brown
PY  - 2020
DA  - 2020/11
TI  - Primary African American Endothelial Cells Exhibit Endothelial Dysfunction with an Exacerbated Inflammatory Profile and Blunted MMP-2 Activity
JO  - Artery Research
SP  - 38
EP  - 46
VL  - 27
IS  - 1
SN  - 1876-4401
UR  - https://doi.org/10.2991/artres.k.201102.005
DO  - https://doi.org/10.2991/artres.k.201102.005
ID  - Cook2020
ER  -