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Volume 12, Issue C, December 2015, Pages 20 - 20

P4.21 MICROCIRCULATION EFFECTS OF OBESITY AND/OR DIET: A PRELIMINARY STUDY IN MICE

Authors
Nicole Di Lascio*1, 2, Francesca Lenzarini4, 2, Cristina Barsanti2, Francesco Stea3, 2, Claudia Kusmic2, Francesco Faita2
1Institute of Life Science, Scuola Superiore Sant’Anna, Pisa, Italy
2Institute of Clinical Physiology, National Research Council, Pisa, Italy
3Department of Internal Medicine, University of Pisa, Pisa, Italy
4Fondazione G. Monasterio CNR, Regione Toscana, Pisa, Italy
Available Online 23 November 2015.
DOI
10.1016/j.artres.2015.10.265How to use a DOI?
Abstract

Obesity is becoming a global epidemic and is associated with cardiovascular disease. Genetic factors play a significant role in the disease etiology but less is known about the interaction between genes and diet composition. This study is aimed to investigate the effect of diet and/or genotype on microcirculation in mice.

Five groups of male mice (28 weeks) were examined using micro-ultrasound (Vevo2100, VisualSonics): 5 wild type mice on standard diet (WT_DS), 7 wild type mice on high-fat diet (WT_HF), 7 OB/+ mice on standard diet (OB/+_SD), 5 OB/+ mice on high-fat diet (OB/+_HF) and 4 OB/OB mice on standard diet (OB/OB_SD). The high-fat diet (45% energy as fat) groups were treated for 18 weeks before US scans. Infrarenal vasculature was imaged using Power-Doppler mode and Pulsed-Wave Doppler signals were acquired at the segmental level; Resistivity Index (RI) and Pulsatility Index (PI) were then assessed.

Both RI and PI were significantly lower in WT_DS than in WT_HF (0.57±0.03vs0.67±0.06 and 0.86±0.04vs1.10±0.09, respectively). The same result was found for the comparison between OB/+_SD and OB/+_HF (0.63±0.06vs0.72±0.04 and 1±0.12vs1.22±0.09, respectively). RI and PI values were significantly different between WT_HF and OB/+_HF mice, while no differences were found for WT_DS-OB/+_DS, WT_HF-OB/OB_SD and OB/+_HF-OB/OB_SD comparisons.

The high-fat diet has effects on the microvasculature of both WT and OB/+ mice. The two genotypes respond differently to the high-fat diet but not to the standard one. Moreover, if treated with high-fat diet, WT and OB/+ animals are not different from OB/OB mice (standard diet) in terms of microcirculation.

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<Previous Article In Issue
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Journal
Artery Research
Volume-Issue
12 - C
Pages
20 - 20
Publication Date
2015/11/23
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.1016/j.artres.2015.10.265How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

risenwbib
TY  - JOUR
AU  - Nicole Di Lascio*
AU  - Francesca Lenzarini
AU  - Cristina Barsanti
AU  - Francesco Stea
AU  - Claudia Kusmic
AU  - Francesco Faita
PY  - 2015
DA  - 2015/11/23
TI  - P4.21 MICROCIRCULATION EFFECTS OF OBESITY AND/OR DIET: A PRELIMINARY STUDY IN MICE
JO  - Artery Research
SP  - 20
EP  - 20
VL  - 12
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2015.10.265
DO  - 10.1016/j.artres.2015.10.265
ID  - DiLascio*2015
ER  -