Porphyromonas gingivalis vesicles reduce MDA-LDL levels and aortic wall thickness in high fat diet induced atherosclerosis rats

Aditya Indra Mahendra; Jonny Karunia Fajar; Harapan Harapan; Teuku Heriansyah; Sumarno Reto Prawiro; Edi Widjajanto; Mohammad Saifur Rohman; Karyono Mintaroem; Budi Susetio Pikir; Yash Prashar

doi:10.1016/j.artres.2018.05.008

<Previous Article In Issue

Next Article In Issue>

Volume 23, Issue C, September 2018, Pages 20 - 27

Porphyromonas gingivalis vesicles reduce MDA-LDL levels and aortic wall thickness in high fat diet induced atherosclerosis rats

Authors

Aditya Indra Mahendra^a^{, b}^{, *}, Jonny Karunia Fajar^c, Harapan Harapan^c, Teuku Heriansyah^d^{, **}, Sumarno Reto Prawiro^e, Edi Widjajanto^f, Mohammad Saifur Rohman^b^{, g}, Karyono Mintaroem^h, Budi Susetio Pikirⁱ^{, ***}, Yash Prashar^j

^aFaculty of Medicine, Brawijaya University, Malang, 65145, Indonesia

^bBrawijaya Cardiovascular Research Center, Brawijaya University, Malang, 65145, Indonesia

^cMedical Research Unit, School of Medicine, Syiah Kuala University, Banda Aceh, 23111, Indonesia

^dDepartment of Cardiology and Vascular Medicine, School of Medicine, Syiah Kuala University, Banda Aceh, 23111, Indonesia

^eDepartment of Microbiology, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia

^fDepartment of Clinical Pathology, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia

^gDepartment of Cardiology and Vascular Medicine, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia

^hDepartment of Anatomic Pathology, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia

ⁱDepartment of Cardiology and Vascular Medicine, Faculty of Medicine, Airlangga University, Surabaya, 60286, Indonesia

^jDepartment of Pharmacology, Rayat Institute of Pharmacy, Distt. S.B.S Nagar, Railmajra, Punjab, 144533, India

^*Corresponding author. Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia. E-mail address: adityaindram@gmail.com (A.I. Mahendra)

^**Corresponding author. E-mail address: teuku_hery@unsyiah.ac.id (T. Heriansyah)

^***Corresponding author. E-mail address: bsp49@fk.unair.ac.id (B.S.Pikir).

Corresponding Authors

Aditya Indra Mahendra, Teuku Heriansyah, Budi Susetio Pikir

Received 22 April 2018, Revised 9 May 2018, Accepted 16 May 2018, Available Online 1 June 2018.

DOI: 10.1016/j.artres.2018.05.008 How to use a DOI?
Keywords: Atherosclerosis; Vaccines; MDA-LDL; Vesicles; Porphyromonas gingivalis
Abstract: Background: Recently, atherosclerosis-associated disease has been reported simultaneously increased. Whereas, to date, no atherosclerosis vaccine is available. Since the epitope mimicry between malondialdehyde low-density lipoprotein (MDA-LDL) and arginine specific epitope gingipain (Rgp) on the Porphyromonas gingivalis vesicles has been reported, it raises an opportunity to employ the potency of P. gingivalis as an atherosclerosis vaccine.

Objective: To evaluate the potency of P. gingivalis vesicles to prevent atherosclerosis, by assessing MDA-LDL level, visceral fat, body weight, and aortic wall thickness, in rats model.

Methods: Five groups of rats (n = 10 per group), three treatment groups, one positive and negative control group were assigned and adapted with high fat diet for 8 weeks. The treatment groups were injected with P. gingivalis vesicles with and without adjuvant with four booster doses. The level of MDA-LDL serum, visceral fat, body weight, and aortic wall thickness were measured in the end of the course.

Results: Our present study found that decreased in MDA-LDL levels (p = 0.037) and aortic wall thickness (p = 0.016) were observed in rats treated with vesicles and adjuvants, but not with vesicles or adjuvants only, compared to negative control. Moreover, MDA-LDL levels in rats immunized with vesicles and adjuvants were significantly lower than healthy rats. However, body weight (p = 0.329 and visceral fat (p = 0.789) were not significantly different in all treatment groups compared to control.

Conclusions: Immunization with P. gingivalis vesicles and adjuvants significantly reduces MDA-LDL level and aortic wall thickness in rats model.
Open Access: This is an open access article distributed under the CC BY-NC license.

Download article (PDF)
View full text (HTML)

<Previous Article In Issue

Next Article In Issue>

Journal: Artery Research
Volume-Issue: 23 - C
Pages: 20 - 27
Publication Date: 2018/06/01
ISSN (Online): 1876-4401
ISSN (Print): 1872-9312
DOI: 10.1016/j.artres.2018.05.008 How to use a DOI?
Open Access: This is an open access article distributed under the CC BY-NC license.

Cite this article

ris enw bib

TY  - JOUR
AU  - Aditya Indra Mahendra
AU  - Jonny Karunia Fajar
AU  - Harapan Harapan
AU  - Teuku Heriansyah
AU  - Sumarno Reto Prawiro
AU  - Edi Widjajanto
AU  - Mohammad Saifur Rohman
AU  - Karyono Mintaroem
AU  - Budi Susetio Pikir
AU  - Yash Prashar
PY  - 2018
DA  - 2018/06/01
TI  - Porphyromonas gingivalis vesicles reduce MDA-LDL levels and aortic wall thickness in high fat diet induced atherosclerosis rats
JO  - Artery Research
SP  - 20
EP  - 27
VL  - 23
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2018.05.008
DO  - 10.1016/j.artres.2018.05.008
ID  - Mahendra2018
ER  -

download .riscopy to clipboard