Artery Research

Volume 23, Issue C, September 2018, Pages 20 - 27

Porphyromonas gingivalis vesicles reduce MDA-LDL levels and aortic wall thickness in high fat diet induced atherosclerosis rats

Authors
Aditya Indra Mahendraa, b, *, Jonny Karunia Fajarc, Harapan Harapanc, Teuku Heriansyahd, **, Sumarno Reto Prawiroe, Edi Widjajantof, Mohammad Saifur Rohmanb, g, Karyono Mintaroemh, Budi Susetio Pikiri, ***, Yash Prasharj
aFaculty of Medicine, Brawijaya University, Malang, 65145, Indonesia
bBrawijaya Cardiovascular Research Center, Brawijaya University, Malang, 65145, Indonesia
cMedical Research Unit, School of Medicine, Syiah Kuala University, Banda Aceh, 23111, Indonesia
dDepartment of Cardiology and Vascular Medicine, School of Medicine, Syiah Kuala University, Banda Aceh, 23111, Indonesia
eDepartment of Microbiology, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia
fDepartment of Clinical Pathology, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia
gDepartment of Cardiology and Vascular Medicine, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia
hDepartment of Anatomic Pathology, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia
iDepartment of Cardiology and Vascular Medicine, Faculty of Medicine, Airlangga University, Surabaya, 60286, Indonesia
jDepartment of Pharmacology, Rayat Institute of Pharmacy, Distt. S.B.S Nagar, Railmajra, Punjab, 144533, India
*Corresponding author. Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia. E-mail address: adityaindram@gmail.com (A.I. Mahendra)
**Corresponding author. E-mail address: teuku_hery@unsyiah.ac.id (T. Heriansyah)
***Corresponding author. E-mail address: bsp49@fk.unair.ac.id (B.S.Pikir).
Corresponding Author
Aditya Indra Mahendra
Received 22 April 2018, Revised 9 May 2018, Accepted 16 May 2018, Available Online 1 June 2018.
DOI
https://doi.org/10.1016/j.artres.2018.05.008How to use a DOI?
Keywords
Atherosclerosis, Vaccines, MDA-LDL, Vesicles, Porphyromonas gingivalis
Abstract

Background: Recently, atherosclerosis-associated disease has been reported simultaneously increased. Whereas, to date, no atherosclerosis vaccine is available. Since the epitope mimicry between malondialdehyde low-density lipoprotein (MDA-LDL) and arginine specific epitope gingipain (Rgp) on the Porphyromonas gingivalis vesicles has been reported, it raises an opportunity to employ the potency of P. gingivalis as an atherosclerosis vaccine.

Objective: To evaluate the potency of P. gingivalis vesicles to prevent atherosclerosis, by assessing MDA-LDL level, visceral fat, body weight, and aortic wall thickness, in rats model.

Methods: Five groups of rats (n = 10 per group), three treatment groups, one positive and negative control group were assigned and adapted with high fat diet for 8 weeks. The treatment groups were injected with P. gingivalis vesicles with and without adjuvant with four booster doses. The level of MDA-LDL serum, visceral fat, body weight, and aortic wall thickness were measured in the end of the course.

Results: Our present study found that decreased in MDA-LDL levels (p = 0.037) and aortic wall thickness (p = 0.016) were observed in rats treated with vesicles and adjuvants, but not with vesicles or adjuvants only, compared to negative control. Moreover, MDA-LDL levels in rats immunized with vesicles and adjuvants were significantly lower than healthy rats. However, body weight (p = 0.329 and visceral fat (p = 0.789) were not significantly different in all treatment groups compared to control.

Conclusions: Immunization with P. gingivalis vesicles and adjuvants significantly reduces MDA-LDL level and aortic wall thickness in rats model.

Copyright
© 2018 Association for Research into Arterial Structure and Physiology. Published by Elsevier B.V. All rights reserved.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Journal
Artery Research
Volume-Issue
23 - C
Pages
20 - 27
Publication Date
2018/06
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
https://doi.org/10.1016/j.artres.2018.05.008How to use a DOI?
Copyright
© 2018 Association for Research into Arterial Structure and Physiology. Published by Elsevier B.V. All rights reserved.
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - JOUR
AU  - Aditya Indra Mahendra
AU  - Jonny Karunia Fajar
AU  - Harapan Harapan
AU  - Teuku Heriansyah
AU  - Sumarno Reto Prawiro
AU  - Edi Widjajanto
AU  - Mohammad Saifur Rohman
AU  - Karyono Mintaroem
AU  - Budi Susetio Pikir
AU  - Yash Prashar
PY  - 2018
DA  - 2018/06
TI  - Porphyromonas gingivalis vesicles reduce MDA-LDL levels and aortic wall thickness in high fat diet induced atherosclerosis rats
JO  - Artery Research
SP  - 20
EP  - 27
VL  - 23
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2018.05.008
DO  - https://doi.org/10.1016/j.artres.2018.05.008
ID  - Mahendra2018
ER  -