Regional Progression on Diffusion and Transport of Aβ Protein in Transgenic Mice Brain

DOI

10.2991/assehr.k.220401.139How to use a DOI?

Keywords

diffusion; APP; Aβ; hippocampus; cortex; transgenic mice

Abstract

Accumulation of amyloid plaques is considered one of the pathological markers of Alzheimer’s disease (AD). In this work, the hypothesis: “The accumulation of amyloid-beta plaque is initiated from the hippocampus and Aβ plaques diffuse to the entire cortex.” is tested by designing two experiments to find out the mechanism of transport and production of amyloid-beta protein, using transgenic mice without familial Alzheimer’s disease (FAD) gene expression in the hippocampus in experiment 1. The second experiment uses transgenic mice with E693Q Dutch mutation of amyloid precursor protein (APP) gene expression in the hippocampus and normal FAD gene expression in the cortical cells. CRISPR Cas-9 gene-editing technology, viral transduction, IP – immunoprecipitation, elusion and HPLC – high-performance liquid chromatography is used in this experiment. The purpose of this paper is to find the transport of amyloid-beta protein to investigate the cause of Alzheimer’s Disease.

Copyright

© 2022 The Authors. Published by Atlantis Press SARL.

Open Access

This is an open access article distributed under the CC BY-NC 4.0 license.

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TY  - CONF
AU  - Yihuan Fu
PY  - 2022
DA  - 2022/04/08
TI  - Regional Progression on Diffusion and Transport of Aβ Protein in Transgenic Mice Brain
BT  - Proceedings of the 2022 International Conference on Social Sciences and Humanities and Arts (SSHA 2022)
PB  - Atlantis Press
SP  - 731
EP  - 735
SN  - 2352-5398
UR  - https://doi.org/10.2991/assehr.k.220401.139
DO  - 10.2991/assehr.k.220401.139
ID  - Fu2022
ER  -